鸡NLRP3单克隆抗体的研制及其抗原表位鉴定
Chicken NLRP3 monoclonal antibody development and its antigenic epitope identification.
作者信息
Xu Mengmeng, Huang Mengmeng, Wang Guodong, Han Jingzhe, Yu Hangbo, Zhang Yulong, Liu Runhang, Wu Ziwen, Cui Hongyu, Zhang Yanping, Wang Suyan, Liu Yongzhen, Chen Yuntong, Duan Yulu, Li Liuan, Gao Yulong, Qi Xiaole
机构信息
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China; Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.
Avian Immunosuppressive Diseases Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, China.
出版信息
Poult Sci. 2025 Aug 6;104(11):105641. doi: 10.1016/j.psj.2025.105641.
Inflammation is key important pathogenic response that occurs in several poultry diseases. NOD-like receptor protein 3 inflammasome plays a central role in initiating inflammation. However, the lack of antibodies against chicken-derived inflammatory factors presents a major bottleneck in related studies. In this study, we developed a monoclonal antibody (mAb) against chicken NLRP3 using hybridoma technology. This antibody can recognize both eukaryotic and prokaryotic NLRP3 proteins as well as the upregulation of endogenous NLRP3 in HD11 cells induced by lipopolysaccharide or infectious bursal disease virus. Furthermore, a novel antigenic epitope, 42DELEKVTHPSS52, located in the PYD domain of chicken NLRP3 and specifically recognized by this mAb was identified. This epitope is unique to chickens and valuable for distinguishing chicken NLRP3 from orthologs in other species. The developed mAb provides an important tool for detecting chicken NLRP3 and facilitates further study of its antigenic structure and biochemical characteristics.
炎症是几种家禽疾病中关键的重要致病反应。NOD样受体蛋白3炎性小体在引发炎症中起核心作用。然而，缺乏针对鸡源炎症因子的抗体是相关研究中的一个主要瓶颈。在本研究中，我们利用杂交瘤技术开发了一种针对鸡NLRP3的单克隆抗体（mAb）。该抗体能够识别真核和原核NLRP3蛋白，以及脂多糖或传染性法氏囊病病毒诱导的HD11细胞中内源性NLRP3的上调。此外，还鉴定出一个位于鸡NLRP3的PYD结构域中的新型抗原表位42DELEKVTHPSS52，该表位可被此单克隆抗体特异性识别。此表位是鸡所特有的，对于区分鸡NLRP3与其他物种的直系同源物具有重要价值。所开发的单克隆抗体为检测鸡NLRP3提供了重要工具，并有助于进一步研究其抗原结构和生化特性。
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