Yao J K, Rastetter G M
Anal Biochem. 1985 Oct;150(1):111-6. doi: 10.1016/0003-2697(85)90447-6.
We have developed a high-performance thin-layer chromatographic method, not involving preliminary column chromatography, for separating mixtures of nonpolar lipids, glycosphingolipids, and phospholipids in one dimension. Three or four consecutive developing solvent systems are used. More than 20 different lipid subclasses can be separated using this procedure. Reproducible results have been obtained by careful control of variables. In addition, a simple and rapid fractionation procedure, using Sep-Pak silica cartridges, has been developed to concentrate neutral glycosphingolipids from biological membranes, e.g., erythrocytes, so that patients with sphingolipidosis can be easily identified.
我们开发了一种高性能薄层色谱法，无需初步柱色谱法，可在一维上分离非极性脂质、糖鞘脂和磷脂的混合物。使用三到四个连续的展开溶剂系统。通过该程序可分离出20多种不同的脂质亚类。通过仔细控制变量获得了可重复的结果。此外，还开发了一种使用Sep-Pak硅胶柱的简单快速分馏程序，以从生物膜（如红细胞）中浓缩中性糖鞘脂，从而能够轻松识别患有鞘脂病的患者。
