Hassid A, Levine L
J Biol Chem. 1977 Oct 10;252(19):6591-3.
Canine kidney cells (MDCK) in which [3H]arachidonic acid was esterified in the cellular lipids released increased levels of radioactive prostaglandins and arachidonic acid into the medium when cultured in the presence of benzo(a)pyrene. When MDCK cells were cultured in the presence of benzo(alpha)pyrene and 7,8-benzoflavone, this increased release was not observed. MDCK cells incubated with benzo(a)pyrene also converted exogenous arachidonic acid into prostaglandins more effectively than cells grown in its absence. 7,8-Benzoflavone inhibited this benzo(a)pyrene effect. Microsomes, prepared from benzo(alpha)pyrene-treated MDCK cells synthesized prostaglandin F2alpha from arachidonic acid more effectively than nontreated cells.
在细胞脂质中[3H]花生四烯酸已被酯化的犬肾细胞（MDCK），当在苯并(a)芘存在的情况下培养时，会使释放到培养基中的放射性前列腺素和花生四烯酸水平增加。当MDCK细胞在苯并(α)芘和7,8 - 苯并黄酮存在的情况下培养时，未观察到这种释放增加的现象。与未接触苯并(a)芘而生长的细胞相比，用苯并(a)芘孵育的MDCK细胞也能更有效地将外源性花生四烯酸转化为前列腺素。7,8 - 苯并黄酮抑制了这种苯并(a)芘效应。从经苯并(α)芘处理的MDCK细胞制备的微粒体，比未处理的细胞更有效地从花生四烯酸合成前列腺素F2α。
