Coyne C P, Hornof W J, Kelly A B, O'Brien T R, DeNardo S J
Am J Vet Res. 1985 Dec;46(12):2578-81.
Two methods were analyzed for the rapid extraction of equine fibrinogen from fresh plasma, using ammonium sulfate-sodium phosphate buffer. Fibrinogen from each of these 2 methods was then radiolabeled with 125I (half-life = 60.2 days, gamma = 35 keV), using monochloroiodine reagent. Mean protein-bound activity was 98.5% and mean clottable radioactivity was 94.1%. Radiolabeled fibrinogen administered IV to 15 horses had an overall mean (+/- SD) plasma half-life of 4.95 +/- 0.44 days.
分析了两种使用硫酸铵-磷酸钠缓冲液从新鲜血浆中快速提取马纤维蛋白原的方法。然后，使用一氯碘试剂，将通过这两种方法提取的纤维蛋白原用¹²⁵I（半衰期=60.2天，γ=35keV）进行放射性标记。平均蛋白结合活性为98.5%，平均可凝放射性为94.1%。对15匹马静脉注射放射性标记的纤维蛋白原后，其血浆半衰期总体平均（±标准差）为4.95±0.44天。
