Tencerova Michaela, Palmisano Biagio, Lucas Stéphanie, Attané Camille, Ivaska Kaisa K, Loisay Léa, Ikushima Yoshiko M, Trivanovic Drenka, Corsi Alessandro, Roque Adriana, Li Hongshuai, Behler-Janbeck Friederike, Geurts Jeroen, Riminucci Mara, Podgorski Izabela, Cawthorn William P, van der Eerden Bram C J, van Wijnen André J
Laboratory of Molecular Physiology of Bone, Institute of Physiology of the Czech Academy of Sciences, Prague, Czechia.
Department of Molecular Medicine, Sapienza University, Rome, Italy.
Bone Rep. 2025 Jul 28;26:101861. doi: 10.1016/j.bonr.2025.101861. eCollection 2025 Sep.
Bone marrow adipose tissue (BMAT) is physiologically linked to bone and energy metabolism, endocrine regulation, hematopoiesis and cancer-related processes. A key challenge in the field is that methods for isolating BMAT or bone marrow adipocytes (BMAds) are variable because there are no widely adopted standardized protocols. To generate awareness of this challenge and to establish uniformity in experimental approaches requiring isolation, storage and characterization of BMAT and BMAds, the Biobanking Working Group of the international Bone Marrow Adiposity Society (BMAS) has previously recommended experimental standards. This paper provides an update on this effort and presents current state-of-the-art methods and technical considerations for isolation and characterization of BMAT and BMAds, including currently available high-throughput omics approaches. This review provides a reference point based on the consensus view of BMAS investigators to support studies on biomedical, biological, biochemical and biophysical questions associated with bone marrow adiposity.
骨髓脂肪组织（BMAT）在生理上与骨骼、能量代谢、内分泌调节、造血作用及癌症相关过程存在联系。该领域的一个关键挑战在于，由于缺乏广泛采用的标准化方案，分离BMAT或骨髓脂肪细胞（BMAds）的方法存在差异。为了让人们认识到这一挑战，并在需要对BMAT和BMAds进行分离、储存及表征的实验方法上实现统一，国际骨髓脂肪协会（BMAS）的生物样本库工作组此前已推荐了实验标准。本文对这一工作进行了更新，并介绍了BMAT和BMAds分离与表征的当前最先进方法及技术考量，包括目前可用的高通量组学方法。本综述基于BMAS研究人员的共识观点提供了一个参考点，以支持有关骨髓肥胖的生物医学、生物学、生物化学和生物物理问题的研究。
