Romain F, Versmisse E, Pescher P, Augier J
Ann Inst Pasteur Microbiol (1985). 1985 Sep-Oct;136B(2):235-48. doi: 10.1016/s0769-2609(85)80048-x.
The preparation of antigen L using culture filtrates of live BCG is described. The procedure consisted essentially of precipitating this filtrate with 10% trichloroacetic acid in the presence of N-butyl alcohol 4% final concentration. The precipitate was chromatographed on carboxy-methyl-trisacryl-M and then on DEAE-trisacryl-M; in both cases, maximal biological activity (delayed-type hypersensitivity) was found in the void volume. The product thus obtained was chromatographed on a molecular sieve so as to eliminate poorly active fractions. The final product was 30-fold more active on guinea-pigs sensitized with live BCG than on those sensitized with inactivated BCG.
描述了使用活卡介苗培养滤液制备抗原L的方法。该程序主要包括在最终浓度为4%的正丁醇存在下，用10%的三氯乙酸沉淀该滤液。沉淀物先后在羧甲基三丙烯酸M和二乙氨基乙基三丙烯酸M上进行色谱分离；在这两种情况下，在空体积中均发现了最大生物活性（迟发型超敏反应）。由此获得的产物在分子筛上进行色谱分离，以去除活性较低的部分。最终产物对用活卡介苗致敏的豚鼠的活性比对用灭活卡介苗致敏的豚鼠高30倍。
