Fontes Patricia Kubo, Silva Ana Beatriz Florencio da, Bartoli Ana Beatriz Dos Reis, Antunes Thays, Júnior Arnaldo Rodrigues Dos Santos, Milazzotto Marcella Pecora
Center of Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil.
Center of Natural and Human Sciences, Federal University of ABC, São Bernardo do Campo, SP, Brazil.
Mol Reprod Dev. 2025 Aug;92(8):e70049. doi: 10.1002/mrd.70049.
In vitro models to study the oviduct are challenged by cellular dedifferentiation, a complex coculture system for embryo production, limited cell lifespan, and/or very complex methodologies. Hence, we aimed to develop an in vitro oviductal model using the magnetic bioprinting system, a three-dimensional (3D) culture system. Using the bovine epithelial and stromal oviductal cells (BOEC and BOSC, respectively), we produced the Oviductal Magnetic Spheroid (OMS), a duo somatic cell spheroid aggregate with self-organization capacity. The OMS showed to be viable for 21 days and recapitulated the oviductal tissue features after 7 days in culture, such as a simple epithelial cell layer facing outwards, expressing ciliation (acetylated tubulin positive) and secretory marker (oviduct-specific glycoprotein 1). Although the responsiveness for hormonal treatment with estradiol and progesterone in an estrous cycle-dependent way might require further improvements, the OMS offers an ethical and practical alternative as a three-dimensional oviductal in vitro model to study oviductal physiology, and maybe, a future platform to test therapies and a technology aiming to improve fertility and assisted reproduction success.
用于研究输卵管的体外模型面临着细胞去分化、用于胚胎生产的复杂共培养系统、有限的细胞寿命和/或非常复杂的方法等挑战。因此，我们旨在利用磁性生物打印系统（一种三维（3D）培养系统）开发一种体外输卵管模型。我们使用牛输卵管上皮细胞和基质细胞（分别为BOEC和BOSC），构建了输卵管磁性球体（OMS），这是一种具有自我组织能力的双体细胞球体聚集体。OMS在培养21天内保持活力，并在培养7天后重现了输卵管组织特征，例如向外的简单上皮细胞层，表达纤毛（乙酰化微管蛋白阳性）和分泌标记物（输卵管特异性糖蛋白1）。尽管以发情周期依赖方式对雌二醇和孕酮激素处理的反应性可能需要进一步改进，但OMS作为一种用于研究输卵管生理学的三维体外输卵管模型，提供了一种符合伦理且实用的替代方案，也许还能成为未来测试治疗方法的平台以及一种旨在提高生育力和辅助生殖成功率技术的平台。
