Wang Zhao, Zhang Zhiming, Guo Lizhen, Miao Ning, Shao Yizhen, Chen Yun, Yuan Zhiliang
College of Life Sciences, Henan Agricultural University, Zhengzhou, 450046, China; Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.
College of Forestry, Henan Agricultural University, Zhengzhou, 450046, China.
J Environ Manage. 2025 Aug 20;393:127025. doi: 10.1016/j.jenvman.2025.127025.
Land-use changes and economic development have degraded the Yellow River wetland (WL), replacing them with forestland (FL), bare land (BL), and cropland (CL). Soil fungi, crucial to nutrient cycling and ecosystem stability, play a vital role in wetland restoration. As ecological restoration efforts, particularly CL-to-WL conversion, gain momentum, understanding fungal diversity and coexistence is essential for effective wetland conservation and management. This study examined fungal communities across different land-use types in the Yellow River wetland using high-throughput sequencing and FunGuild functional predictions. Fungal diversity, community composition, functional diversity, and network stability were compared across land types. Ascomycota dominated all land-use types, with the lowest diversity in CL and the lowest abundance in BL. Saprotrophic fungi were the most abundant functional group, with WL exhibiting the highest functional diversity. Specialists and generalists responded differently to soil physicochemical properties, with stochastic processes primarily shaping community assembly. Among deterministic processes, heterogeneous selection had a stronger impact on specialists. Network analyses showed the most intensive fungal interactions in BL, where specialists played a key role in maintaining network stability across land-use types. This study examines how fungal communities respond to environmental factors and interspecific interactions, emphasizing the profound impact of land-use changes on fungal diversity and functional diversity. It also highlights the critical role of specialists in maintaining network stability. These findings improve our understanding of land-use effects on fungal communities and provide a scientific basis for informed wetland conservation and management strategies.
土地利用变化和经济发展已使黄河湿地退化，取而代之的是林地、裸地和农田。土壤真菌对养分循环和生态系统稳定性至关重要，在湿地恢复中发挥着关键作用。随着生态恢复工作，特别是农田向湿地的转化不断推进，了解真菌多样性和共存情况对于有效的湿地保护和管理至关重要。本研究利用高通量测序和真菌功能预测，对黄河湿地不同土地利用类型的真菌群落进行了研究。比较了不同土地类型的真菌多样性、群落组成、功能多样性和网络稳定性。子囊菌门在所有土地利用类型中占主导地位，农田中的多样性最低，裸地中的丰度最低。腐生真菌是最丰富的功能类群，湿地的功能多样性最高。专性真菌和泛性真菌对土壤理化性质的反应不同，随机过程主要塑造群落组装。在确定性过程中，异质性选择对专性真菌的影响更强。网络分析表明，裸地中真菌相互作用最为密集，专性真菌在维持不同土地利用类型的网络稳定性方面发挥着关键作用。本研究考察了真菌群落如何响应环境因素和种间相互作用，强调了土地利用变化对真菌多样性和功能多样性的深远影响。它还突出了专性真菌在维持网络稳定性方面的关键作用。这些发现增进了我们对土地利用对真菌群落影响的理解，并为明智的湿地保护和管理策略提供了科学依据。
