Pommerening K, Ristau O, Rein H, Dautzenberg H, Loth F
Biomed Biochim Acta. 1983;42(7-8):813-23.
Cytochrome c, hemoglobin, urease and liver microsomes were microencapsulated in aqueous solution by use of a new method. The membrane of the microcapsules consist of a symplex, which is formed predominantly by electrostatic interactions between a polymeric polyanion and a polymeric polycation. The membrane is characterized by high mechanical stability and is a barrier for globular substances with molecular weights larger than 12000, but permeable for substances with lower molecular weight. The microcapsules contain the protein or microsomes and additionally the polymeric polyanion in the liquid interior. Structure and function of microencapsulated proteins and microsomes are preserved. The developed method of microencapsulation opens new possibilities for the application of biomacromolecules, particularly for extracorporal detoxification.
采用一种新方法在水溶液中对细胞色素c、血红蛋白、脲酶和肝微粒体进行了微囊化。微囊的膜由一种复合物构成，该复合物主要通过聚合聚阴离子和聚合聚阳离子之间的静电相互作用形成。该膜具有高机械稳定性，是分子量大于12000的球状物质的屏障，但对分子量较低的物质具有渗透性。微囊中含有蛋白质或微粒体，此外在液体内部还含有聚合聚阴离子。微囊化蛋白质和微粒体的结构和功能得以保留。所开发的微囊化方法为生物大分子的应用开辟了新的可能性，特别是在体外解毒方面。
