Jia Yujiao, Han Lijuan, Hou Shengzhen, Gui Linsheng, Yuan Zhenzhen, Sun Shengnan, Wang Zhiyou, Yang Baochun, Yang Chao
College of Agriculture and Animal Husbandry, Qinghai University, Xining, China.
Front Microbiol. 2025 Aug 5;16:1573040. doi: 10.3389/fmicb.2025.1573040. eCollection 2025.
This study aimed to investigate the effects of natural forage from different regions (saline-alkali and non-saline-alkali areas) on the rumen microbiota, muscle metabolites, and meat quality of Tibetan sheep.
Targeted and non-targeted metabolomics were used to comprehensively analyze both pasture and meat quality, metabolites. Additionally, 16S rDNA sequencing was employed to analyze the rumen microbial community structure of Tibetan sheep.
The results showed that the natural saline-alkali forage (HG group) had higher protein content, lower fiber content, higher relative feed value, and better quality. Metabolomic analysis revealed significant accumulation of flavonoids and upregulation of amino acid metabolism in the HG group. Additionally, the natural saline-alkali forage significantly increased amino acid deposition in Tibetan sheep muscle, markedly enhanced the redness value (a*), and significantly reduced the yellowness value (b*). Furthermore, the natural saline-alkali forage altered the rumen fermentation patterns in Tibetan sheep, leading to a significant increase in the abundance of F082 and WCHB1-41, while significantly reducing the abundance of Prevotellaceae_UCG-003. Correlation analysis revealed that these microbial taxa were significantly influenced by the natural saline-alkali forage,while also showing significant associations with muscle quality parameters (a*, b*) and metabolites (cysteine, C18:1n9, etc.).
Overall, the natural saline-alkali forage demonstrated superior quality and metabolite content compared to natural non-saline-alkali forage. Furthermore, this saline-alkali forage significantly influenced the abundance of specific rumen microbiota in Tibetan sheep, consequently regulating muscle coloration and amino acid deposition.
本研究旨在探讨来自不同地区（盐碱地和非盐碱地）的天然草料对藏羊瘤胃微生物群、肌肉代谢产物和肉质的影响。
采用靶向和非靶向代谢组学方法全面分析草料和肉质、代谢产物。此外，采用16S rDNA测序分析藏羊瘤胃微生物群落结构。
结果表明，天然盐碱地草料（HG组）蛋白质含量较高，纤维含量较低，相对饲料价值较高，质量较好。代谢组学分析显示，HG组黄酮类化合物显著积累，氨基酸代谢上调。此外，天然盐碱地草料显著增加了藏羊肌肉中氨基酸的沉积，显著提高了红度值（a*），并显著降低了黄度值（b*）。此外，天然盐碱地草料改变了藏羊的瘤胃发酵模式，导致F082和WCHB1-41的丰度显著增加，而Prevotellaceae_UCG-003的丰度显著降低。相关性分析表明，这些微生物类群受天然盐碱地草料的显著影响，同时也与肌肉品质参数（a*、b*）和代谢产物（半胱氨酸、C18:1n9等）显著相关。
总体而言，与天然非盐碱地草料相比，天然盐碱地草料具有更优的品质和代谢产物含量。此外，这种盐碱地草料显著影响了藏羊瘤胃中特定微生物群的丰度，从而调节了肌肉色泽和氨基酸沉积。
