College of Forestry and Prataculture, Ningxia University, Yinchuan 750021, China.
Agricultural Biotechnology Research Center, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China.
J Agric Food Chem. 2024 Mar 13;72(10):5477-5490. doi: 10.1021/acs.jafc.3c07139. Epub 2024 Feb 28.
is a saline-alkali-tolerant plant whose aerial parts are rich in flavonoids; however, the role of these flavonoids in saline-alkali tolerance remains unclear. Herein, we performed physiological, metabolomics, and transcriptomics analyses in leaves under alkaline salt stress for different durations. Alkaline salt stress stimulated excessive accumulation of reactive oxygen species and consequently destroyed the cell membrane, causing cell death, and initiated osmotic regulation and the antioxidant system to respond to stress. In total, 803 metabolites, including 244 flavonoids, were detected via metabolomics analysis. Differentially altered metabolites and differentially expressed genes were coenriched in flavonoid-related pathways. Genes such as novel.4890, Glyur001511s00039602, and Glyur000775s00025737 were highly expressed, and flavonoid metabolites such as 2'-hydroxygenistein, apigenin, and 3--methylquercetin were upregulated. Thus, flavonoids as nonenzymatic antioxidants play an important role in stress tolerance. These findings provide novel insights into the response of to alkaline salt stress.
是一种耐盐碱性植物，其地上部分富含类黄酮；然而，这些类黄酮在耐盐碱性中的作用尚不清楚。在此，我们对不同时间碱盐胁迫下的叶片进行了生理、代谢组学和转录组学分析。碱盐胁迫刺激活性氧的过度积累，进而破坏细胞膜，导致细胞死亡，并启动渗透调节和抗氧化系统来应对胁迫。通过代谢组学分析共检测到 803 种代谢物，包括 244 种类黄酮。差异改变的代谢物和差异表达的基因在类黄酮相关途径中共同富集。novel.4890、Glyur001511s00039602 和 Glyur000775s00025737 等基因高度表达，2'-hydroxygenistein、芹菜素和 3--甲基槲皮素等类黄酮代谢物上调。因此，类黄酮作为非酶抗氧化剂在胁迫耐受性中发挥重要作用。这些发现为 对碱性盐胁迫的响应提供了新的见解。
