Hei Jingying, Li Yue, Rui Rui, Faisal Noor, Peng Jiansong, Wang Biao, Wang Shu, He Xiahong
College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming, China.
Department of Biochemistry and Molecular Biology, School of Life Sciences, China Medical University, Shenyang, China.
Front Microbiol. 2025 Aug 6;16:1616266. doi: 10.3389/fmicb.2025.1616266. eCollection 2025.
The species of tree most appropriate for the cultivation of Sanqi in an understory environment is pine. Nevertheless, the precise type of pine that confers the greatest benefit to soil health during Sanqi cultivation has not been definitively established.
Herein, four distinct land use configurations were established, including the , , Sanqi- (SPA), and Sanqi- (SPK) systems. High-throughput sequencing technology and metabolomics analysis were used to comparatively evaluate variations in bacterial and fungal community structures and soil metabolites between the SPA and SPK systems.
After cultivating Sanqi, the content of total phosphorus, ammonium nitrogen, and total potassium as well as water content and soil pH were significantly increased in soil. Moreover, the bacterial and fungal copy numbers, alpha- and beta-diversity, remained unchanged in the soil of , but significantly decreased in the soil of following Sanqi planting. Moreover, Sanqi cultivation significant increased complexity of the microbial network in rather than soil, while the network stability was maintained. Structural equation modeling indicated that soil enzymes, metabolites, and edaphic factors enhanced the complexity of the microbial network in soil in SPA system. Additionally, the content of eight differentially accumulated metabolites (DAMs) was significantly increased in the rhizosphere and bulk soils of . In conclusion, the cultivation of Sanqi benefits the microbiome and metabolites in rather than soil, thus providing an important theoretical foundation for the sustainable development of Sanqi cultivation.
在林下环境中最适合三七种植的树种是松树。然而，在三七种植过程中对土壤健康最有益的具体松树种类尚未明确确定。
在此，建立了四种不同的土地利用配置，包括 、 、三七 - （SPA）和三七 - （SPK）系统。采用高通量测序技术和代谢组学分析，比较评估SPA和SPK系统之间细菌和真菌群落结构以及土壤代谢物的差异。
种植三七后， 土壤中总磷、铵态氮和总钾的含量以及含水量和土壤pH值均显著增加。此外， 土壤中的细菌和真菌拷贝数、α - 和β - 多样性保持不变，但在种植三七后 土壤中的细菌和真菌拷贝数、α - 和β - 多样性显著降低。此外，三七种植显著增加了 而非 土壤中微生物网络的复杂性，同时维持了网络稳定性。结构方程模型表明，土壤酶、代谢物和土壤因子增强了SPA系统中 土壤中微生物网络的复杂性。此外， 根际和土壤中八种差异积累代谢物（DAMs）的含量显著增加。总之，三七种植对 而非 土壤中的微生物群落和代谢物有益，从而为三七种植的可持续发展提供了重要的理论基础。
