伊拉克乳腺癌患者谷胱甘肽S-转移酶Pi 1（GSTP1）基因多态性的可能关联
Possible Association of Glutathione S-Transferase Pi 1 (GSTP1) Gene Polymorphism in Iraqi Patients with Breast Cancer.
作者信息
Alhayali Hasan, Hojati Zohreh
机构信息
Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Sciences and Technology, University of Isfahan, Iran.
出版信息
Rep Biochem Mol Biol. 2025 Jan;13(4):516-524. doi: 10.61186/rbmb.13.4.516.
BACKGROUND
Investigating the role of glutathione S-transferase pi 1 (GSTP1) in breast cancer development and exploring genetic variations in GSTP1 that may contribute to susceptibility to the disease.
METHODS
Blood samples were collected from 40 healthy control individuals and 75 breast cancer patients for genomic DNA extraction. PCR and bioinformatics analysis were used to examine the GSTP1 gene sequences.
RESULTS
Gel electrophoresis confirmed the presence of a 433-bp amplified genetic locus with a 212C>A variation in the intron-4 region, identified as rs757152293. The Single nucleotide polymorphisms (SNP) exhibited variable distributions, with homozygous CC and heterozygous CA genotypes. Patient samples with the CA genotype were submitted to NCBI under accession numbers OL957029-OL957036, while those with the CC genotype were submitted under accession numbers OL957027-OL957034. Additionally, sequence analysis of the rs757152293 SNP in healthy individuals was submitted to NCBI under accession numbers OL957037-OL957041.
CONCLUSIONS
This study is the first to suggest a possible association between GSTP1 genetic polymorphism and breast cancer in the investigated population.
背景
研究谷胱甘肽S-转移酶pi 1（GSTP1）在乳腺癌发生中的作用，并探索GSTP1基因变异，这些变异可能与该疾病的易感性有关。
方法
采集40名健康对照个体和75名乳腺癌患者的血样用于基因组DNA提取。采用聚合酶链反应（PCR）和生物信息学分析检测GSTP1基因序列。
结果
凝胶电泳证实存在一个433 bp的扩增基因座，在内含子4区域存在212C>A变异，被鉴定为rs757152293。单核苷酸多态性（SNP）呈现出不同的分布，有纯合CC和杂合CA基因型。CA基因型的患者样本以登录号OL957029 - OL957036提交至美国国立生物技术信息中心（NCBI），而CC基因型的样本以登录号OL957027 - OL957034提交。此外，健康个体中rs757152293 SNP的序列分析以登录号OL957037 - OL957041提交至NCBI。
结论
本研究首次表明在被调查人群中GSTP1基因多态性与乳腺癌之间可能存在关联。
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