Degawa M, Kojima M, Masuko T, Hishinuma T, Hashimoto Y
Biochem Biophys Res Commun. 1985 Dec 31;133(3):1072-7. doi: 10.1016/0006-291x(85)91245-8.
Treatment of Sprague Dawley rats with 3-methoxy-4-aminoazobenzene (3-MeO-AAB) resulted in striking increase of the activity of hepatic microsomal cytochrome P-450s which could efficiently catalyze the mutagenic activation of hepatocarcinogenic aromatic amines such as a tryptophan-pyrolysate component, Trp P-2, and a glutamic acid-pyrolysate component, Glu P-1. The 3-MeO-AAB-induced cytochrome P-450 (3-MeO-AAB-P-450) was examined for the molecular character by immuno-Western blotting using monoclonal antibody to 3-methylcholanthrene-induced cytochrome P-448 (P-448H; m.w. 54,000).
用3-甲氧基-4-氨基偶氮苯（3-MeO-AAB）处理斯普拉格-道利大鼠，导致肝微粒体细胞色素P-450s的活性显著增加，这些细胞色素P-450s能够有效地催化致癌芳香胺的诱变活化，如色氨酸裂解产物Trp P-2和谷氨酸裂解产物Glu P-1。使用针对3-甲基胆蒽诱导的细胞色素P-448（P-448H；分子量54,000）的单克隆抗体，通过免疫蛋白质印迹法检测3-MeO-AAB诱导的细胞色素P-450（3-MeO-AAB-P-450）的分子特征。
