Degawa M, Yamada H, Hishinuma T, Masuko T, Hashimoto Y
Department of Hygienic Chemistry, Tohoku University, Miyagi.
J Biochem. 1987 Jun;101(6):1437-45. doi: 10.1093/oxfordjournals.jbchem.a122013.
Male Sprague Dawley rats were injected intraperitoneally with 2-methoxy-4-amino-azobenzene (2-MeO-AAB) or 3-methylcholanthrene (MC), and then the expression of microsomal cytochrome P-450 isozymes in liver and extrahepatic tissues was investigated by means of immunological methods and a bacterial mutation test. The results of protein A-enzyme-linked immunosorbent assaying and immunoblotting using anti-rat cytochrome P-448 monoclonal antibodies showed that MC induced at least two microsomal cytochrome P-448 isozymes, a high spin form (cytochrome P-448H) and a low spin form (cytochrome P-448L), in liver, but that it induced only cytochrome P-448L in extrahepatic tissues such as lung, kidney, small intestine, and colon. The results also indicated that, in contrast to MC, 2-MeO-AAB selectively induced microsomal cytochrome P-448H in liver but did not induce any cytochrome P-448 isozymes in extrahepatic tissues. The activities of 9,000 X g supernatants from the individual organs, as to the mutagenic conversion of 3 aromatic amines (3-amino-1-methyl-5H-pyrido(4,3-b)indole, 2-amino-6-methyldipyrido(1,2-a: 3',2'-d)-imidazole and 3-methoxy-4-aminoazobenzene), toward Salmonella typhimurium TA 98 bacteria were dependent upon the quantity and/or quality of the microsomal cytochrome P-448 isozymes in the organs.
将雄性斯普拉格-道利大鼠腹腔注射2-甲氧基-4-氨基偶氮苯（2-MeO-AAB）或3-甲基胆蒽（MC），然后通过免疫学方法和细菌突变试验研究肝脏和肝外组织中微粒体细胞色素P-450同工酶的表达。使用抗大鼠细胞色素P-448单克隆抗体进行蛋白A-酶联免疫吸附测定和免疫印迹的结果表明，MC在肝脏中诱导至少两种微粒体细胞色素P-448同工酶，一种高自旋形式（细胞色素P-448H）和一种低自旋形式（细胞色素P-448L），但在肺、肾、小肠和结肠等肝外组织中仅诱导细胞色素P-448L。结果还表明，与MC相反，2-MeO-AAB在肝脏中选择性诱导微粒体细胞色素P-448H，但在肝外组织中不诱导任何细胞色素P-448同工酶。各器官9000×g上清液对3种芳香胺（3-氨基-1-甲基-5H-吡啶并(4,3-b)吲哚、2-氨基-6-甲基二吡啶并(1,2-a:3',2'-d)-咪唑和3-甲氧基-4-氨基偶氮苯）向鼠伤寒沙门氏菌TA 98细菌的诱变转化活性取决于器官中微粒体细胞色素P-448同工酶的数量和/或质量。
