迷迭香提取物：植物化学成分及消除多微生物生物膜和多重耐药细菌的潜力。

Rosemary Extract: Phytochemical Composition and Potential for Eliminating Polymicrobial Biofilm of and Multidrug-Resistant Bacteria.

作者信息

Cintra Tuana Mendonça Faria, Menezes Raquel Teles de, de Carvalho Lara Steffany, de Miguel Nazario Leticia, Hantao Leandro Wang, Marcucci Maria Cristina, Oliveira Luciane Dias de, Meccatti-Domiciano Vanessa Marques

机构信息

Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (ICT-UNESP), São José dos Campos 12245-000, Brazil.

Institute of Chemistry-University of Campinas LCGC Separations Group, Campinas 13083-862, Brazil.

出版信息

BioTech (Basel). 2025 Aug 13;14(3):61. doi: 10.3390/biotech14030061.

DOI:10.3390/biotech14030061

PMID:40843784

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12372108/

Abstract

Herbal medicines can be promising for the treatment of infections caused by multidrug-resistant microorganisms. This study aimed to evaluate (Rosemary) hydroalcoholic extract (RHE) regarding its phytochemical composition and potential for eliminating polymicrobial biofilm of with multidrug-resistant bacteria (, , and ). The extraction and quantification of the extract (flavonoids and phenols) were performed, and its antioxidant activity (DPPH) and the presence of bio-active compounds were investigated using high-performance liquid chromatography with Diode Array Detection (HPLC-DAD) and Gas Chromatography-Mass Spectrometry (GC-MS). The minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) were determined, and the extract's action on polymicrobial biofilms was evaluated using the MTT assay. Data were analyzed using one-way ANOVA and Tukey's tests, as well as Kruskal-Wallis and Dunn's tests, with a significance level of 5%. RHE showed compatible amounts of flavonoids and phenols, with an EC50 of 19.53 µg/mL. Through HPLC-DAD and GC-MS, biomolecules such as rosmarinic acid and α-Pinene were identified. The extract exhibited microbicidal activity and antibiofilm action, with reduction percentages of up to 69.6% ( < 0.05), showing superior performance compared to 0.12% chlorhexidine against + . In conclusion, RHE may be a promising therapeutic agent against multidrug-resistant pathogens.

摘要

草药对于治疗由多重耐药微生物引起的感染可能很有前景。本研究旨在评估迷迭香水醇提取物（RHE）的植物化学成分及其消除由多重耐药细菌（金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌）形成的混合生物膜的潜力。对提取物（黄酮类化合物和酚类）进行了提取和定量，并使用带有二极管阵列检测的高效液相色谱法（HPLC-DAD）和气相色谱-质谱联用仪（GC-MS）研究了其抗氧化活性（DPPH）和生物活性化合物的存在情况。测定了最低抑菌浓度（MIC）和最低杀菌浓度（MMC），并使用MTT法评估了提取物对混合生物膜的作用。数据采用单因素方差分析和Tukey检验，以及Kruskal-Wallis和Dunn检验进行分析，显著性水平为5%。RHE显示出适量的黄酮类化合物和酚类，其半数有效浓度（EC50）为19.53μg/mL。通过HPLC-DAD和GC-MS鉴定出了迷迭香酸和α-蒎烯等生物分子。该提取物表现出杀菌活性和抗生物膜作用，减少百分比高达69.6%（P<0.05），与0.12%的氯己定相比，对金黄色葡萄球菌+大肠杆菌显示出更优的性能。总之，RHE可能是一种有前景的抗多重耐药病原体治疗剂。