Hol P R, van Andel A C, van Ederen A M, Draaÿer J, Gruys E
Br J Exp Pathol. 1985 Dec;66(6):689-97.
An amyloid enhancing factor (AEF) was extracted from spleens and livers of casein-treated hamsters. It shortened the induction time of experimental amyloidosis in recipient hamsters from 14 days to 4 days. Sepharose-4B gel filtration resolved the extract into 4 different fractions, with molecular weights of: higher than IO(7) (Vo-fraction), about 280 000, 59 000 and 12 000 respectively. In all 4 fractions AEF was present, indicating that AEF is probably a low molecular weight substance that easily aggregates, or associates with other compounds present in the spleen extract. AEF was precipitable at 50% ammonium sulphate saturation. On anion exchange chromatography in PBS (pH 7.2) of this precipitate, AEF was found in the fraction eluting at high NaCl concentration. This fraction did not show any relation to hamster protein AA, IgG or albumin with double immunodiffusion. Ultraviolet absorption spectrophotometry indicated nucleotide-like material, suggesting AEF to be related to nucleoproteins.
从经酪蛋白处理的仓鼠的脾脏和肝脏中提取出一种淀粉样增强因子（AEF）。它将受体仓鼠实验性淀粉样变性的诱导时间从14天缩短至4天。琼脂糖-4B凝胶过滤将提取物分离为4种不同组分，分子量分别为：高于10⁷（空体积组分）、约280000、59000和12000。所有4个组分中均存在AEF，这表明AEF可能是一种低分子量物质，容易聚集，或与脾脏提取物中存在的其他化合物结合。AEF在50%硫酸铵饱和度下可沉淀。对该沉淀物在PBS（pH 7.2）中进行阴离子交换色谱分析时，在高NaCl浓度洗脱的组分中发现了AEF。通过双向免疫扩散法，该组分与仓鼠蛋白AA、IgG或白蛋白均无关联。紫外吸收分光光度法表明存在类核苷酸物质，提示AEF与核蛋白有关。
