Choovanichvong Wararak, Supa-Amornkul Sirirak, Rungraungrayabkul Dulyapong, Boonyapratheeprat Natcha, Meenetkum Sasiwimon, Boongird Sarinya, Chuengsaman Piyatida, Kitiyakara Chagriya, Sangkhamanee Sujiwan Seubbuk
Department of Oral Medicine and Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
PLoS One. 2025 Aug 22;20(8):e0330767. doi: 10.1371/journal.pone.0330767. eCollection 2025.
To investigate salivary microbiota composition in end-stage kidney disease (ESKD) patients with sarcopenia (SESKD), ESKD patients without sarcopenia (NSESKD), and individuals without chronic kidney disease (control group).
Thirty-three participants were enrolled: 10 SESKD patients, 12 NSESKD patients, and 11 controls. Demographic data, oral examinations, and unstimulated saliva samples were collected. Salivary bacterial microbiomes were analyzed using high-throughput sequencing targeting the V3-V4 region of the bacterial 16S rRNA gene.
The overall bacterial abundance and distribution were significantly higher in the SESKD and NSESKD groups compared to the control group (p < 0.05), with no significant differences between the SESKD and NSESKD groups. ESKD, educational level, and muscle strength were significantly associated with variations in the salivary microbiome composition. Analysis of bacterial abundance revealed a shift in trends as the disease combined from control to NSESKD and SESKD group, respectively, across 7 genera: Actinobacillus, TM7x, Capnocytophaga, Neisseria, and Leptotrichia increased in abundance, while Actinomyces and Atopobium decreased. Linear discriminant analysis effect size (LEfSe) identified Leptotrichia as a potential biomarker for ESKD (both with and without sarcopenia).
ESKD condition impacted microbial composition, with minimal influence from sarcopenia. Specifically, Leptotrichia was notably higher in the ESKD group.
研究患有肌肉减少症的终末期肾病（ESKD）患者、无肌肉减少症的ESKD患者以及无慢性肾病个体（对照组）的唾液微生物群组成。
招募了33名参与者：10名患有肌肉减少症的ESKD患者、12名无肌肉减少症的ESKD患者和11名对照者。收集了人口统计学数据、口腔检查结果和非刺激性唾液样本。使用针对细菌16S rRNA基因V3-V4区域的高通量测序分析唾液细菌微生物群。
与对照组相比，患有肌肉减少症的ESKD组和无肌肉减少症的ESKD组的总体细菌丰度和分布显著更高（p < 0.05），患有肌肉减少症的ESKD组和无肌肉减少症的ESKD组之间无显著差异。ESKD、教育水平和肌肉力量与唾液微生物群组成的变化显著相关。细菌丰度分析显示，随着疾病从对照组分别合并到无肌肉减少症的ESKD组和患有肌肉减少症的ESKD组，7个菌属的趋势发生了变化：放线杆菌属、TM7x、嗜二氧化碳噬纤维菌属、奈瑟菌属和纤毛菌属的丰度增加，而放线菌属和阿托波菌属减少。线性判别分析效应大小（LEfSe）确定纤毛菌属为ESKD（无论有无肌肉减少症）的潜在生物标志物。
ESKD状况影响微生物组成，肌肉减少症的影响最小。具体而言，纤毛菌属在ESKD组中明显更高。
