Blake K R, Murakami A, Spitz S A, Glave S A, Reddy M P, Ts'o P O, Miller P S
Biochemistry. 1985 Oct 22;24(22):6139-45. doi: 10.1021/bi00343a016.
Oligodeoxyribonucleoside methylphosphonates which are complementary to the 5' end, the initiation codon regions, or the coding regions of rabbit globin mRNA were synthesized. These oligomers were shown to interact with their complementary mRNA binding sites by their ability to serve as primers for reverse transcriptase. In several cases, the priming efficiency of the oligomers was enhanced when the oligomer was preannealed with the mRNA. This behavior correlates with the predicted secondary structure of the mRNA and suggests that some oligomer binding sites occur in hydrogen-bonded stem regions of the mRNA. Methylphosphonate oligomers inhibit translation of globin mRNA in reticulocyte lysates. Inhibition is due to the interaction of the oligomers with mRNA. The extent of inhibition is affected by the sequence and chain length of the oligomer, the location of the oligomer binding site on the mRNA, and the secondary structure of the binding site. Oligomers which bind to the 5' end and initiation codon regions of beta-globin mRNA inhibit both alpha- and beta-globin synthesis whereas oligomers which bind to the coding region of alpha-globin mRNA or the coding region of beta-globin mRNA inhibit translation of their target mRNA in a specific manner. Oligodeoxyribonucleoside methylphosphonates inhibit globin synthesis in rabbit reticulocytes. The effects of various oligomers on cellular globin synthesis are similar to those in the lysate system and suggest that the conformation of globin mRNA is the same in both systems during translation.
合成了与兔珠蛋白mRNA的5′端、起始密码子区域或编码区域互补的寡脱氧核糖核苷甲基膦酸酯。这些寡聚物通过作为逆转录酶引物的能力，显示出与它们的互补mRNA结合位点相互作用。在几种情况下，当寡聚物与mRNA预退火时，寡聚物的引发效率会提高。这种行为与mRNA预测的二级结构相关，表明一些寡聚物结合位点出现在mRNA的氢键茎区。甲基膦酸酯寡聚物抑制网织红细胞裂解物中珠蛋白mRNA的翻译。抑制是由于寡聚物与mRNA的相互作用。抑制程度受寡聚物的序列和链长、寡聚物在mRNA上的结合位点位置以及结合位点的二级结构影响。与β-珠蛋白mRNA的5′端和起始密码子区域结合的寡聚物抑制α-和β-珠蛋白的合成，而与α-珠蛋白mRNA编码区域或β-珠蛋白mRNA编码区域结合的寡聚物以特定方式抑制其靶mRNA的翻译。寡脱氧核糖核苷甲基膦酸酯抑制兔网织红细胞中的珠蛋白合成。各种寡聚物对细胞珠蛋白合成的影响与裂解物系统中的相似，表明在翻译过程中，珠蛋白mRNA在两个系统中的构象相同。
