Hagerman P J
Biochemistry. 1985 Dec 3;24(25):7033-7. doi: 10.1021/bi00346a001.
Certain DNA molecules derived from both prokaryotic and eukaryotic organisms display markedly abnormal electrophoretic behavior on polyacrylamide gels. These molecules share a common element of sequence which involves collections of A/T residues that are approximately in phase with the helix repeat. This sequence periodicity has led to the suggestion that such phasing is important in generating the abnormal behavior. We have demonstrated that such phasing is, in fact, essential, thus ruling out alternative models which invoke any form of isotropic or centrosymmetric flexibility as the source of the phenomenon. We have also shown that the abnormal behavior is not a simple consequence of marginal thermodynamic stability. The most plausible explanation for the observed behavior is that stable, local distortions of the helix axis result in macroscopic curvature when such distortions are propagated in phase with the helix repeat.
某些源自原核生物和真核生物的DNA分子在聚丙烯酰胺凝胶上表现出明显异常的电泳行为。这些分子共享一个共同的序列元素，该元素涉及与螺旋重复大致同相的A/T残基集合。这种序列周期性引发了这样的推测，即这种相位在产生异常行为中很重要。我们已经证明，事实上这种相位是必不可少的，从而排除了以任何形式的各向同性或中心对称柔韧性作为该现象来源的替代模型。我们还表明，异常行为并非边缘热力学稳定性的简单结果。对观察到的行为最合理的解释是，当这种扭曲与螺旋重复同相传播时，螺旋轴的稳定局部扭曲会导致宏观曲率。
