Whitnall M H, Gainer H
Brain Res. 1985 Dec 30;361(1-2):400-4. doi: 10.1016/0006-8993(85)91312-5.
It has been proposed that neurosecretory material may be transported in a non-vesicular compartment in magnocellular neurons in the hypothalamus of osmotically stimulated rats. We have reexamined this issue using postembedding electron microscopic immunoperoxidase labeling of the secreted peptides (vasopressin and neurophysin) found in these neurons. Reaction product was found exclusively over neurosecretory vesicles in cell bodies, and in axons in the median eminence and neurohypophysis. The findings are consistent with an exclusive secretory vesicle location of the neuropeptides in the hypothalamo-neurohypophysial system of both normal and dehydrated rats.
有人提出，在渗透性刺激的大鼠下丘脑大细胞神经元中，神经分泌物质可能在非囊泡区室中运输。我们使用对这些神经元中发现的分泌肽（血管加压素和神经垂体素）进行包埋后电子显微镜免疫过氧化物酶标记的方法，重新审视了这个问题。反应产物仅在细胞体中的神经分泌囊泡上以及正中隆起和神经垂体的轴突中被发现。这些发现与正常和脱水大鼠下丘脑 - 神经垂体系统中神经肽仅位于分泌囊泡中的情况一致。
