Shaw F D, Castel M, Morris J F
Cell Tissue Res. 1987 Aug;249(2):403-10. doi: 10.1007/BF00215524.
Synapses in the lateral septum of the murine brain have been investigated by ultrastructural immunocytochemistry, using monoclonal anti-neurophysins in both immunoperoxidase and immunogold techniques. In the region shown by light microscopy to be rich in vasopressinergic innervation, synaptic boutons containing approximately 30 nm clear vesicles and occasional approximately 100 nm dense-cored granules (granules) were stained by pre-embedding immunoperoxidase procedures with antisera to vasopressin-neurophysin, but not oxytocin-neurophysin; reaction product was diffusely distributed in the terminals. Terminals were symmetrical, and both axosomatic and axodendritic in type. Postembedding immunogold procedures by use of anti-vasopressin-neurophysin labeled only the approximately 100 nm diameter granules in the terminals. Sodium meta-periodate treatment 'bleached' immunoreactive granules, indicating the presence of a carbohydrate residue. The quantum of peptide packaged in the granules appears to be smaller than that in magnocellular neurones; nevertheless, the results indicate that, as in the magnocellular neurosecretory system, vasopressin and its neurophysin are packaged exclusively in granules, and that vasopressin in the septum is likely to be derived from a precursor comprising vasopressin, vasopressin-neurophysin and a glycosylated residue.
利用免疫过氧化物酶和免疫金技术中的单克隆抗神经垂体素，通过超微结构免疫细胞化学方法对小鼠脑外侧隔中的突触进行了研究。在光学显微镜显示富含加压素能神经支配的区域，含有约30nm清亮小泡和偶尔约100nm致密核心颗粒（颗粒）的突触小体，用抗加压素-神经垂体素抗血清的包埋前免疫过氧化物酶程序染色，但不用抗催产素-神经垂体素染色；反应产物弥散分布于终末。终末是对称的，有轴体和轴树突两种类型。使用抗加压素-神经垂体素的包埋后免疫金程序仅标记终末中直径约100nm的颗粒。高碘酸钠处理使免疫反应性颗粒“褪色”，表明存在碳水化合物残基。颗粒中包装的肽量似乎比大细胞神经元中的少；然而，结果表明，与大细胞神经分泌系统一样，加压素及其神经垂体素仅包装在颗粒中，并且隔区中的加压素可能来自包含加压素、加压素-神经垂体素和糖基化残基的前体。
