Macknight A D
J Membr Biol. 1977 Aug 18;36(1):55-63. doi: 10.1007/BF01868143.
Epithelial cells were scraped from the bladders of toads of the species Bufo marinus obtained from the Dominican Republic. These epithelial cells exchanged their chloride virtually completely with 36Cl i; the medium within 60 min. Of this chloride, about 93% came from the serosal medium. The approximately 20 mmole/kg dry wt of chloride which equilibrates with 36Cl in the mucosal medium was still present when choline replaced sodium in the medium in the presence of amiloride (10(-4) M) and was almost all readily removed by rapid washing of the mucosal surface immediately prior to analysis. These observations suggest that little chloride of mucosal origin is truly intracellular. This conclusion is supported by the fact that after vasopressin the increased cellular chloride was not of mucosal origin.
从多米尼加共和国获取的海蟾蜍膀胱中刮取上皮细胞。这些上皮细胞在60分钟内几乎完全将其氯离子与36Cl i进行了交换；其中约93%的氯离子来自浆膜介质。当在存在氨氯地平（10(-4) M）的情况下用胆碱替代介质中的钠时，与36Cl在黏膜介质中平衡的约20毫摩尔/千克干重的氯离子仍然存在，并且在分析前通过快速冲洗黏膜表面几乎全部很容易被去除。这些观察结果表明，几乎没有黏膜来源的氯离子真正存在于细胞内。血管加压素作用后细胞内增加的氯离子并非来自黏膜，这一事实支持了这一结论。
