Wen Xiang, Feng Kaiyang, Wei Peng, Li Jinhang, Li Ming, Dou Wei, Guo Zhaojiang, Zhang Youjun, He Lin
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River, Ministry of Education, Chongqing, China.
Commun Biol. 2025 Aug 23;8(1):1274. doi: 10.1038/s42003-025-08726-0.
Arthropods have evolved advanced metabolic detoxification pathways that have helped them adapt to complex stresses induced by plant secondary metabolites and synthetic pesticides. Nonetheless, how xenobiotics induce the suite of intracellular metabolic changes important for detoxification in arthropods remains unexplored. In this study, we found that the ancient oxidative stress signaling pathway has adapted to transmit the detoxification signal in T. cinnabarinus, where accumulation of reactive oxygen species (ROS) plays a crucial role in initiating detoxification metabolism. Acaricides exposure led to ROS accumulation, resulting in the activation of Forkhead box O (FOXO) that in turn upregulates the expression of downstream xenobiotic sensors, including CncC, AhR, and HR96. These xenobiotic sensors then upregulate the expression of downstream detoxification genes, helping mites metabolically detoxify acaricides. This study reveals a cascade-amplifying mechanism for broad-spectrum detoxification in T. cinnabarinus that is initiated through ROS-FOXO triggering broad-spectrum detoxification of xenobiotics. These findings further elucidate the adaptive evolution of arthropods and suggest new avenues for novel pesticides and pest control.
节肢动物已经进化出先进的代谢解毒途径，这些途径帮助它们适应由植物次生代谢物和合成农药诱导的复杂压力。然而，异生物质如何诱导节肢动物体内对解毒至关重要的一系列细胞内代谢变化仍未得到探索。在本研究中，我们发现古老的氧化应激信号通路已经适应在朱砂叶螨中传递解毒信号，其中活性氧（ROS）的积累在启动解毒代谢中起关键作用。杀螨剂暴露导致ROS积累，从而激活叉头框O（FOXO），进而上调下游异生物质传感器（包括CncC、AhR和HR96）的表达。这些异生物质传感器随后上调下游解毒基因的表达，帮助螨虫对杀螨剂进行代谢解毒。本研究揭示了朱砂叶螨中通过ROS-FOXO触发异生物质广谱解毒的级联放大机制。这些发现进一步阐明了节肢动物的适应性进化，并为新型农药和害虫防治提供了新途径。
