Ishida R, Kozaki M, Takahashi T
Cell Struct Funct. 1985 Dec;10(4):405-9. doi: 10.1247/csf.10.405.
Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damaging agents has not yet been elucidated. During studies on the effect of caffeine on DNA repair, we found by alkaline elution analysis that caffeine alone produced DNA strand breaks or alkali labile sites in Chinese hamster ovary cells. The amount of DNA breakage or alkali labile sites depended on the concentration of caffeine. We propose that DNA breakage induced by caffeine may be involved in the enhancement of the lethal effect of DNA-damaging agents.
咖啡因已被证明可增强DNA损伤剂对哺乳动物细胞的致死效应，而咖啡因对这种效应的增强作用通常被解释为咖啡因抑制了受损DNA的修复。然而，咖啡因增强DNA损伤剂致死效应的机制尚未阐明。在研究咖啡因对DNA修复的影响过程中，我们通过碱性洗脱分析发现，咖啡因单独作用时会在中国仓鼠卵巢细胞中产生DNA链断裂或碱不稳定位点。DNA断裂或碱不稳定位点的数量取决于咖啡因的浓度。我们认为，咖啡因诱导的DNA断裂可能与DNA损伤剂致死效应的增强有关。
