Starkey J R, Hosick H L
J Natl Cancer Inst. 1977 Sep;59(3):859-66. doi: 10.1093/jnci/59.3.859.
A rat liver cell line, Lew A1, was isolated from W/LEW rats. It had the normal female karyotype in the lower passage numbers, but in the higher passages it was aneuploid. This line was passaged 65 times, produced rat serum albumin, and consisted of an apparently homogeneous population of typical epithelial cells. The cells also had high levels of the inducible aryl hydrocarbon hydroxylase enzyme complex. A series of experiments described here defined the normal clonal behavior of this line and its modification by repeated treatments with a carcinogenic polycyclic hydrocarbon, 7,12-dimethylbenz[a]anthracene. The results were discussed with particular reference to metastasis, preneoplastic changes, and neoplastic progression.
一种大鼠肝细胞系Lew A1是从W/LEW大鼠中分离出来的。在较低传代次数时它具有正常的雌性核型，但在较高传代时它是非整倍体。该细胞系传代了65次，能产生大鼠血清白蛋白，并且由一群明显均一的典型上皮细胞组成。这些细胞还具有高水平的可诱导芳烃羟化酶复合物。此处描述的一系列实验确定了该细胞系的正常克隆行为以及用致癌多环烃7,12-二甲基苯并[a]蒽反复处理对其的改变。特别参照转移、癌前变化和肿瘤进展对结果进行了讨论。
