Wei Yucai, Zhang Jiaxing, Li Xiaomei, Zhu Weijing, Zhang Peng, Cheng Huijuan, Chen Qian, Wen Zhongjie, Liu Huanhuan, Zhao Lei, Li Yumin
Cancer Center, The Second Hospital of Lanzhou University, Lanzhou, China.
Gansu Province Key Laboratory of Environmental Oncology, The Second Hospital of Lanzhou University, 82 Cuiyingmen, Lanzhou, 730030, Gansu, China.
Sci Rep. 2025 Aug 25;15(1):31168. doi: 10.1038/s41598-025-16610-4.
Gastric cancer (GC) is a prevalent malignancy and one of the leading causes of cancer-related mortality worldwide. The role of transmembrane channel-like 5 (TMC5) in GC remains unknown. In this study, we analyzed the expression of TMC5 in GC using a multi-faceted approach, including analysis of public databases, immunohistochemical assays, and Western blot analyses. Our results demonstrated that TMC5 was overexpressed in GC tissues and cell lines, which was associated with poor prognosis and various clinical characteristics. We observed that the mutation rates of TMC5 in GC were low, and its methylation status was inversely related to its expression levels. Furthermore, functional assays demonstrated that TMC5 promoted GC cell proliferation, migration, and invasion in vitro, as well as tumor growth and metastasis in vivo. Furthermore, the TMC5 expression level was positively correlated with stemness and chemotherapy resistance in GC. Additionally, TMC5 was associated with multiple immune cell infiltration levels, immunotherapy response and immune checkpoint genes. Subsequently, the co-expression genes of TMC5 in GC were screened and verified by qRT-PCR. Gene set enrichment analysis showed that these genes were mainly involved in biosynthesis, metabolic pathways, and multiple classic cancer-related signaling pathways. In conclusion, our study reveals the important role of TMC5 in GC and provides potential new targets and strategies for the diagnosis and treatment of GC.
胃癌（GC）是一种常见的恶性肿瘤，也是全球癌症相关死亡的主要原因之一。跨膜通道样蛋白5（TMC5）在胃癌中的作用尚不清楚。在本研究中，我们采用多方面的方法分析了TMC5在胃癌中的表达，包括对公共数据库的分析、免疫组织化学检测和蛋白质印迹分析。我们的结果表明，TMC5在胃癌组织和细胞系中过表达，这与预后不良和各种临床特征相关。我们观察到胃癌中TMC5的突变率较低，其甲基化状态与其表达水平呈负相关。此外，功能分析表明，TMC5在体外促进胃癌细胞增殖、迁移和侵袭，在体内促进肿瘤生长和转移。此外，TMC5表达水平与胃癌的干性和化疗耐药性呈正相关。此外，TMC5与多种免疫细胞浸润水平、免疫治疗反应和免疫检查点基因有关。随后，通过qRT-PCR筛选并验证了胃癌中TMC5的共表达基因。基因集富集分析表明，这些基因主要参与生物合成、代谢途径以及多种经典的癌症相关信号通路。总之，我们的研究揭示了TMC5在胃癌中的重要作用，并为胃癌的诊断和治疗提供了潜在的新靶点和策略。
