Wang Jingtao, Li Benyue, Zhang Yuanman, Ma Wei, Jia Ting, Yang Jie, Zhang Yexiang, Qi Fengwei, Jia Yan, Song Liya
Beijing Key Laboratory of Plant Resources Research and Development, Department of Cosmetics, School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, China.
Shandong Huahuitang Biotechnology Co., Ltd., Shanghai, China.
Front Microbiol. 2025 Aug 7;16:1504054. doi: 10.3389/fmicb.2025.1504054. eCollection 2025.
The composition of the skin microbiota is shaped by the interaction of multiple factors, with area-specific changes and physiological characteristics in the skin having the most profound impact. The back of the hand and lower leg are two dry areas of human skin. Whether their microbial compositions are consistent, as well as the changes in skin microbiota at these two areas among individuals with dry skin, warrant further discussion.
Using 16S rRNA sequencing, we analyzed the differences of microbiota in dry skin areas of 54 young women and their changes in dry populations. Concurrently, key physiological parameters (Hydration, TEWL, sebum secretion) were measured.
Analysis of physiological parameters showed that Hydration, TEWL and sebum secretion were significantly lower ( < 0.05) in the lower leg compared to the back of the hand. Hydration was significantly lower ( < 0.05) at the same skin area in the dry-skinned population compared to the healthy population. Regarding microbial composition, the relative abundance of Firmicutes and Proteobacteria was significantly higher on the lower leg compared to the back of the hand, whereas the relative abundance of Actinobacteriota was notably greater on the back of the hand than on the lower leg (P < 0.05). Compared to the lower leg, the back of the hand showed a higher relative abundance of (34.19% vs. 8.68%), whereas the lower leg was predominantly colonized by (17% vs. 13.76%). At the genus level, the relative abundance of was significantly increased in the dry skin group, whereas a decreasing trend was observed for . Redundancy analysis (RDA) showed that Streptococcus was negatively correlated with Hydration, TEWL, and sebum, and vice versa for .
These findings suggest that differences in skin microbiota are primarily influenced by a combination of skin area micro environmental factors and not solely dependent on dryness status, suggesting that area-specific ecological niche design should be taken into account when conducting clinical interventions.
皮肤微生物群的组成受多种因素相互作用的影响，其中皮肤特定区域的变化和生理特征影响最为深远。手背和小腿是人体皮肤的两个干燥区域。它们的微生物组成是否一致，以及干性皮肤个体这两个区域的皮肤微生物群变化，值得进一步探讨。
我们采用16S rRNA测序分析了54名年轻女性干性皮肤区域微生物群的差异及其在干性人群中的变化。同时，测量了关键生理参数（水合作用、经皮水分流失、皮脂分泌）。
生理参数分析表明，与手背相比，小腿的水合作用、经皮水分流失和皮脂分泌显著更低（P<0.05）。与健康人群相比，干性皮肤人群相同皮肤区域的水合作用显著更低（P<0.05）。关于微生物组成，与手背相比，小腿上厚壁菌门和变形菌门的相对丰度显著更高，而放线菌门在手背上的相对丰度明显高于小腿（P<0.05）。与小腿相比，手背显示出更高的相对丰度（34.19%对8.68%），而小腿主要由[具体菌属未明确]定殖（17%对13.76%）。在属水平上，干性皮肤组[具体菌属未明确]的相对丰度显著增加，而[具体菌属未明确]呈下降趋势。冗余分析（RDA）表明，链球菌与水合作用、经皮水分流失和皮脂呈负相关，反之亦然。
这些发现表明，皮肤微生物群的差异主要受皮肤区域微环境因素的综合影响，而不仅仅取决于干燥状态，这表明在进行临床干预时应考虑特定区域的生态位设计。
