Fukumoto Y, Tsumoto K, Tashiro A, Ohtomo N, Yoshida T
Complement. 1985;2(2-3):140-6. doi: 10.1159/000467854.
Serum depleted of the first component of complement (C1D) was prepared by treating fresh human serum with Sepharose-IgG in the presence of triethylenetetramine-N,N,N',N'',N''',N'''-hexa-acetic acid and di-isopropyl fluorophosphate at acidic pH (5.2). The total hemolytic activity of this C1D could be increased to approximately 75% of that of the original serum by the addition of excess purified C1. A linear relation was obtained on determination of C1 hemolytic activity using C1D, and the C1 titers of sera from patients measured by this simple method showed a good correlation with those measured using intermediate cells.
通过在酸性pH（5.2）条件下，用琼脂糖-免疫球蛋白（Sepharose-IgG）处理新鲜人血清，并加入三亚乙基四胺-N,N,N',N'',N''',N'''-六乙酸和二异丙基氟磷酸，制备出缺乏补体第一成分（C1D）的血清。通过添加过量纯化的C1，这种C1D的总溶血活性可提高至原始血清的约75%。使用C1D测定C1溶血活性时得到线性关系，用这种简单方法测量的患者血清C1滴度与使用中间细胞测量的结果显示出良好的相关性。
