Ni Suna, Zhao Qian, Hu Fengjiao
Department of Obstetrics Clinic, Shijiazhuang Maternal and Child Health Hospital, No. 396, Youyi South Street, Qiaoxi District, Shijiazhuang, 050000, Hebei, China.
Blood Collection Room, Shijiazhuang Maternal and Child Health Hospital, Shijiazhuang, 050000, Hebei, China.
Ir J Med Sci. 2025 Aug 25. doi: 10.1007/s11845-025-04042-y.
To investigate the diagnostic value and mechanism of action of miR-326 in gestational diabetes mellitus (GDM).
The study population consisted of 98 GDM patients and 71 healthy pregnant women. Subjects' serum were collected early in pregnancy (10-12 weeks) for cryopreservation. Gene expression was analyzed by RT-qPCR. miR-326 was evaluated by ROC for its diagnostic value. Pearson correlation analysis was performed to calculate the relationship between miR-326 and glycemic index and TLR4. Logistic analysis resulted in risk factors for adverse pregnancy outcomes. CCK8, transwell, and flow cytometry were performed to observe changes in trophoblast function after different treatments. DLR verified the targeting relationship between genes.
miR-326 is upregulated in early pregnancy serum of GDM patients and has a predictive diagnostic value for GDM. There is a positive correlation between miR-326 and glycemic indices (HbA1c, FBG, HOMA-IR) in GDM patients, which is a risk factor for inducing adverse pregnancy outcomes. miR-326 inhibitor restored the proliferation, migration, and invasion ability of high glucose-treated (HG group) cells and reduced apoptosis. TLR4 is a target gene of miR-326, and the expression of the two is negatively correlated in GDM patients. Transfection of si-TLR4 resisted the changes in trophoblast cells induced by the use of miR-326 inhibitor.
High levels of miR-326 are upregulated in serum of GDM patients in early pregnancy. miR-326 targeted inhibition of TLR4 causes abnormal trophoblast cell function and induces adverse pregnancy outcomes in GDM patients.
探讨miR-326在妊娠期糖尿病（GDM）中的诊断价值及作用机制。
研究人群包括98例GDM患者和71例健康孕妇。在妊娠早期（10 - 12周）采集受试者血清进行冷冻保存。通过RT-qPCR分析基因表达。采用ROC评估miR-326的诊断价值。进行Pearson相关性分析以计算miR-326与血糖指数及TLR4之间的关系。进行Logistic分析得出不良妊娠结局的危险因素。采用CCK8、transwell和流式细胞术观察不同处理后滋养层细胞功能的变化。通过双荧光素酶报告基因实验（DLR）验证基因之间的靶向关系。
miR-326在GDM患者妊娠早期血清中上调，对GDM具有预测诊断价值。GDM患者中miR-326与血糖指标（糖化血红蛋白、空腹血糖、胰岛素抵抗指数）呈正相关，是诱导不良妊娠结局的危险因素。miR-326抑制剂可恢复高糖处理（HG组）细胞的增殖、迁移和侵袭能力，并减少细胞凋亡。TLR4是miR-326的靶基因，二者在GDM患者中的表达呈负相关。转染si-TLR4可抵抗miR-326抑制剂对滋养层细胞的影响。
GDM患者妊娠早期血清中miR-326水平上调。miR-326靶向抑制TLR4导致滋养层细胞功能异常，诱导GDM患者出现不良妊娠结局。
