采用细胞壁分级分离程序分析植物细胞中五氯苯酚的不可提取（结合）残留物。

Analysis for nonextractable (bound) residues of pentachlorophenol in plant cells using a cell wall fractionation procedure.

作者信息

Langebartels C, Harms H

出版信息

Ecotoxicol Environ Saf. 1985 Oct;10(2):268-79. doi: 10.1016/0147-6513(85)90073-9.

Abstract

When plant cell cultures or aseptically grown wheat plants were treated with [14C]-pentachlorophenol (PCP) a major part of the label was found in a nonextractable or "bound" residue fraction. Soluble polar conjugates participated in the formation of these residues which were mainly located in the plant cell walls. By a sequential fractionation procedure using enzymatic and chemical methods, 90 to 95% of the bound radioactivity could be attributed to individual cell wall components. The 14C label from PCP was found mainly in hemicellulose, lignin, and protein fractions. Associations of hemicellulose with PCP derivatives with molecular weights up to 500,000 were purified to constant specific radioactivity. Hydrolysis of this fraction released 32% PCP and other unidentified products.

摘要

当用[¹⁴C] - 五氯苯酚（PCP）处理植物细胞培养物或无菌培养的小麦植株时，大部分放射性标记物存在于不可提取或“结合”的残留部分中。可溶性极性共轭物参与了这些残留物的形成，这些残留物主要位于植物细胞壁中。通过使用酶法和化学方法的连续分级分离程序，90%至95%的结合放射性可归因于单个细胞壁成分。PCP中的¹⁴C标记主要存在于半纤维素、木质素和蛋白质部分。分子量高达500,000的PCP衍生物与半纤维素的结合物被纯化至恒定的比放射性。该部分的水解释放出32%的PCP和其他未鉴定产物。