Sandermann H, Scheel D, vdTrenck T
Ecotoxicol Environ Saf. 1984 Apr;8(2):167-82. doi: 10.1016/0147-6513(84)90059-9.
The metabolism of the following environmental chemicals has been studied in cell suspension cultures of wheat (Triticum aestivum L.) and soybean (Glycine max L.):2, 4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), hexachlorobenzene, pentachlorophenol, diethylhexylphthalate , benzo [alpha] pyrene, and DDT. All chemicals tested, including the persistent ones, were partially metabolized. Polar conjugates predominated in all cases. A covalent incorporation into lignin could be demonstrated for 2,4-D and pentachlorophenol. A specific deposition in the cellular vacuole could be demonstrated for the beta-D-glucopyranoside conjugates derived from 2,4-D. A rapid assay procedure to evaluate the metabolism of a given 14C-labeled chemical in plant cell suspension cultures is described. This procedure requires about 1 week, and the reproducibility of the results obtained has been assessed.
以下环境化学物质在小麦（普通小麦）和大豆（大豆）的细胞悬浮培养物中的代谢情况已得到研究：2,4-二氯苯氧乙酸（2,4-D）、2,4,5-三氯苯氧乙酸（2,4,5-T）、六氯苯、五氯苯酚、邻苯二甲酸二（2-乙基己基）酯、苯并[a]芘和滴滴涕。所有测试的化学物质，包括持久性化学物质，都有部分被代谢。在所有情况下，极性共轭物占主导。对于2,4-D和五氯苯酚，可以证明其共价结合到木质素中。对于源自2,4-D的β-D-吡喃葡萄糖苷共轭物，可以证明其在细胞液泡中的特异性沉积。描述了一种评估给定14C标记化学物质在植物细胞悬浮培养物中代谢的快速测定方法。该方法大约需要1周时间，并且已评估了所得结果的重现性。
