Chen Xianjun, Jiang Yao, Zhang Jianwei, Liu Xiaocheng, Wang Lulu, Zheng Jintong, Zeng Jiayu, Yang Qin, Zhou Yan
Provincial famous teacher Yang Qin studio/Key Laboratory of Molecular Breeding and Variety Creation of Horticultural Plants for Mountain Features in Guizhou Province, School of Life and Health Science, Kaili University, Kaili, China.
Life Science and Technology School, Lingnan Normal University, Zhanjiang, China.
NPJ Sci Food. 2025 Aug 25;9(1):188. doi: 10.1038/s41538-025-00553-1.
Okra fruit rapidly undergoes chemical deterioration after harvest, necessitating effective preservation strategies. This study represents the first comprehensive investigation combining transcriptomic analysis with biochemical assessment to elucidate how SNPs and MT affect antioxidant capacity and chlorophyll metabolism in okra. MT + SNP treatment most effectively preserved fruit quality by reducing weight loss, maintaining color parameters, decreasing oxidative stress markers (HO, MDA), and enhancing antioxidant enzyme activities (SOD, CAT, POD, APX). This treatment stabilized chlorophyll content by modulating degrading enzymes (CLH, PPH, MDcase). Transcriptome analysis revealed 1927 differentially expressed genes associated with antioxidant defense and chlorophyll metabolism. Key antioxidant genes (PODs, GSTs, MDHARs) were upregulated, while chlorophyll metabolism genes (POR, PAO, Lhcbs) showed coordinated expression, maintaining pigment stability. Network analysis identified transcription factors (NAC86, ERF4, MYB24) linking these pathways. This study provides molecular insights for developing postharvest technologies that extend shelf life while maintaining nutritional quality and reducing food waste.
秋葵果实采后迅速发生化学劣变，因此需要有效的保鲜策略。本研究首次将转录组分析与生化评估相结合，以阐明单核苷酸多态性（SNPs）和褪黑素（MT）如何影响秋葵的抗氧化能力和叶绿素代谢。MT+SNP处理通过减少失重、维持颜色参数、降低氧化应激标志物（羟基自由基、丙二醛）以及增强抗氧化酶活性（超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶），最有效地保持了果实品质。该处理通过调节降解酶（叶绿素酶、脱镁叶绿酸水解酶、苹果酸脱氢酶）来稳定叶绿素含量。转录组分析揭示了1927个与抗氧化防御和叶绿素代谢相关的差异表达基因。关键抗氧化基因（过氧化物酶、谷胱甘肽S-转移酶、苹果酸脱氢酶还原酶）上调，而叶绿素代谢基因（叶绿素原脱植基酶、脱镁叶绿酸氧化酶、叶绿素b结合蛋白）呈现协同表达，维持色素稳定性。网络分析确定了连接这些途径的转录因子（NAC86、乙烯应答因子4、MYB24）。本研究为开发延长货架期同时保持营养品质和减少食物浪费的采后技术提供了分子见解。
