伤害感受器对损伤的翻译组学反应的RNA测序数据集

RNA Sequencing Dataset of Nociceptor Translatomic Response to Injury.

作者信息

Hale Christine M, Beauchemin Kyle J, Brann Courtney L, Moulton Julie K, Geguchadze Ramaz, Harrison Benjamin J, Ganter Geoffrey K

机构信息

College of Arts and Sciences, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USA.

College of Osteopathic Medicine, University of New England, 11 Hills Beach Road, Biddeford, ME 04103, USA.

出版信息

Data (Basel). 2025 Feb;10(2). doi: 10.3390/data10020011. Epub 2025 Jan 21.

DOI:10.3390/data10020011

PMID:40855845

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12377072/

Abstract

To prepare to address the mechanisms of injury-induced nociceptor sensitization, we sequenced the translatome of the nociceptors of injured larvae and those of uninjured larvae. Third-instar larvae expressing a green fluorescent protein (GFP)-tagged ribosomal subunit specifically in Class 4 dendritic arborization neurons, recognized as pickpocket-expressing primary nociceptors, via the GAL4/UAS method, were injured by ultraviolet light or sham-injured. Larvae were subjected to translating ribosome affinity purification for the GFP tag and nociceptor-specific ribosome-bound RNA was sequenced.

摘要

为了准备研究损伤诱导的伤害感受器致敏机制，我们对受伤幼虫和未受伤幼虫的伤害感受器的翻译组进行了测序。通过GAL4/UAS方法，在4类树突状分支神经元中特异性表达绿色荧光蛋白（GFP）标记核糖体亚基的三龄幼虫，被认为是表达扒手蛋白的初级伤害感受器，用紫外线照射致伤或假伤。对幼虫进行GFP标签的翻译核糖体亲和纯化，并对伤害感受器特异性核糖体结合的RNA进行测序。