College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, 730070, China.
College of Life Science and Engineering, Northwest Minzu University, Lanzhou, 730030, China.
Sci Rep. 2019 Feb 13;9(1):1963. doi: 10.1038/s41598-018-37898-5.
LncRNA play important roles in regulation of host immune and inflammation responses in defending bacterial infection. Clostridium perfringens (C. perfringens) type C is one of primary bacteria leading to piglet diarrhea and other intestinal inflammatory diseases. For the differences of host immune capacity, individuals usually show resistance and susceptibility to bacterial infection. However, whether and how lncRNAs involved in modulating host immune resistance have not been reported. We have investigated the expression patterns of ileum lncRNAs of 7-day-old piglets infected by C. perfringens type C through RNA sequencing. A total of 16 lncRNAs and 126 mRNAs were significantly differentially expressed in resistance (IR) and susceptibility (IS) groups. Many lncRNAs and mRNAs were identified to regulate resistance and susceptibility of piglets through immune related pathways. Five lncRNAs may have potential function on regulating the expressions of cytokines, these lncRNAs and cytokines work together to co-regulated piglet immune response to C. perfringens, affecting host resistance and susceptibility. These results provide valuable information for understanding the functions of lncRNA and mRNA in affecting piglet diarrhea resistance of defensing to C. perfringens type C, these lncRNAs and mRNAs may be used as the important biomarkers for decreasing C. perfringens spread and diseases in human and piglets.
长链非编码 RNA 在宿主免疫和炎症反应的调节中发挥重要作用，以抵御细菌感染。产气荚膜梭菌（C.perfringens）C 型是导致仔猪腹泻和其他肠道炎症性疾病的主要细菌之一。由于宿主免疫能力的差异，个体通常对细菌感染表现出抵抗力和易感性。然而，是否以及如何通过长链非编码 RNA 来调节宿主免疫抵抗尚未报道。我们通过 RNA 测序研究了感染 C.perfringens C 型的 7 日龄仔猪回肠长链非编码 RNA 的表达模式。在抗性（IR）和易感性（IS）组中，共有 16 个长链非编码 RNA 和 126 个 mRNAs 表达显著差异。许多长链非编码 RNA 和 mRNAs 通过免疫相关途径被鉴定为调节仔猪的抗性和易感性。5 个长链非编码 RNA 可能具有调节细胞因子表达的潜在功能，这些长链非编码 RNA 和细胞因子共同调节仔猪对产气荚膜梭菌的免疫反应，影响宿主的抗性和易感性。这些结果为理解长链非编码 RNA 和 mRNA 影响仔猪抵抗产气荚膜梭菌 C 型腹泻的功能提供了有价值的信息，这些长链非编码 RNA 和 mRNAs 可作为减少产气荚膜梭菌在人和仔猪中传播和疾病的重要生物标志物。
