Chen Lifang, Chen Jiaqi, Lei Ying, Lin Pengcheng, Cao Donglin, Xiao Wei, Hu Liangshan
Guangdong Provincial Key Laboratory On Functional Soft Condensed Matter, Materials and Energy School, Guangdong University of Technology, Panyu District, Guangzhou, 510006, China.
Department of Laboratory Medicine, The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, 510317, China.
Anal Bioanal Chem. 2025 Sep;417(23):5171-5186. doi: 10.1007/s00216-025-06054-0. Epub 2025 Aug 26.
Interleukin-6 (IL-6) is an acute phase response protein. Its level rapidly increases in infection, trauma, and acute inflammatory states. It usually appears earlier than other inflammatory markers such as C-reactive protein and procalcitonin. Therefore, detecting abnormal IL-6 levels (The clinical reference range is greater than 7 pg mL) faster can help humans receive timely treatment and recover their health. This article successfully synthesized a medium-entropy oxide nanoenzyme. It does not require the involvement of hydrogen peroxide and has a low Michaelis constant of 0.101 mM. Based on the interaction between La-based medium-entropy oxide (MEO-La) nanoenzyme and secondary antibody, specific detection of IL-6 in enzyme-linked immunosorbent assay is achieved. The concentration of IL-6 in human serum can be qualitatively detected. The detection limit and linear range are 0.03 pg mL and 5-1000 pg mL, respectively. This work not only is of great significance for better understanding the unique properties of medium-entropy nanoenzymes but also has enormous potential for biochemical sensing of human health.
白细胞介素-6（IL-6）是一种急性期反应蛋白。其水平在感染、创伤和急性炎症状态下会迅速升高。它通常比其他炎症标志物如C反应蛋白和降钙素原出现得更早。因此，更快地检测到异常的IL-6水平（临床参考范围大于7 pg/mL）有助于人们及时接受治疗并恢复健康。本文成功合成了一种中熵氧化物纳米酶。它不需要过氧化氢的参与，米氏常数低至0.101 mM。基于镧基中熵氧化物（MEO-La）纳米酶与二抗之间的相互作用，实现了酶联免疫吸附测定中对IL-6的特异性检测。可定性检测人血清中IL-6的浓度。检测限和线性范围分别为0.03 pg/mL和5 - 1000 pg/mL。这项工作不仅对于更好地理解中熵纳米酶的独特性质具有重要意义，而且在人类健康的生化传感方面具有巨大潜力。
