一种新型综合方法：植物介导的银纳米颗粒合成及其对乳腺癌和细菌治疗的体外和计算机模拟评估

A Novel Integrated Approach: Plant-Mediated Synthesis, in vitro and in silico Evaluation of Silver Nanoparticles for Breast Cancer and Bacterial Therapies.

作者信息

Alghamdi Sahar S, Alhaidal Haifa A, Mohammed Afrah E, Alsubait Arwa, Alshammari Maali D, Alsaqer Lamis, Alzahrani Shahad Saleem, Alanazi Fai, Al Tuhayni Layan Basel, Ali Rizwan, Alharbi Shaden, Hazazi Bader, AlEnazi Mona E, Alehaideb Zeyad I

机构信息

College of Pharmacy (COP), King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia.

Medical Research Core Facility and Platforms, King Abdullah International Medical Research Center (KAIMRC), Ministry of National Guard - Health Affairs, Riyadh, Saudi Arabia.

出版信息

Int J Nanomedicine. 2025 Aug 19;20:10043-10071. doi: 10.2147/IJN.S516723. eCollection 2025.

DOI:10.2147/IJN.S516723

PMID:40859954

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12374718/

Abstract

BACKGROUND AND AIM

Nanotechnology offers a promising approach to address breast cancer and bacterial resistance, two critical global health challenges. This study synthesized silver nanoparticles (AgNPs) using extracts from ( (. and ( to evaluate their potential as novel therapeutic agents.

MATERIALS AND METHODS

AgNP synthesis was achieved via co-precipitation, and their characterization was performed using various techniques. Cytotoxicity was assessed using the MTT assay against multiple breast cancer cell lines (KAIMRC2, MDA-MB231, MCF-7) and a non-malignant control (MCF-10A). Antimicrobial activity was evaluated using a well diffusion assay against and . LC-MS identified several bioactive metabolites, which were further analyzed in silico computational analysis to predict their anti-cancer and antibacterial properties.

RESULTS

The resulting AgNPs demonstrated significant cytotoxicity against breast cancer cells with minimal toxicity to normal cells, and potent antibacterial activity. Specifically, .-AgNPs showed strongest activity against MDA-MB-231, .-AgNPs against MCF-7, and .-AgNPs against KAIMRC-2. Promising lead metabolites, including 1-Acetylaspidospermidine, Apigenin-7-o-glucoside, and Chlorogenic acid, were identified, suggesting potential for development as novel oral anti-cancer and antibacterial agents.

CONCLUSION

Further research focusing on the optimization and preclinical development of these identified metabolites is warranted to explore their translational potential as oral anti-cancer and antibacterial agents.

摘要

背景与目的

纳米技术为应对乳腺癌和细菌耐药性这两个全球重大健康挑战提供了一种很有前景的方法。本研究利用[植物名称1]、[植物名称2]的提取物合成了银纳米颗粒（AgNPs），以评估其作为新型治疗剂的潜力。

材料与方法

通过共沉淀法合成AgNPs，并采用多种技术对其进行表征。使用MTT法评估对多种乳腺癌细胞系（KAIMRC2、MDA - MB231、MCF - 7）和非恶性对照（MCF - 10A）的细胞毒性。使用平板扩散法评估对[细菌名称1]和[细菌名称2]的抗菌活性。液相色谱 - 质谱联用（LC - MS）鉴定了几种生物活性代谢物，并通过计算机模拟分析进一步预测它们的抗癌和抗菌特性。

结果

所得AgNPs对乳腺癌细胞显示出显著的细胞毒性，对正常细胞毒性最小，且具有强大的抗菌活性。具体而言，[AgNP种类1] - AgNPs对MDA - MB - 231显示出最强活性，[AgNP种类2] - AgNPs对MCF - 7，[AgNP种类3] - AgNPs对KAIMRC - 2。鉴定出了有前景的先导代谢物，包括1 - 乙酰阿马里新、芹菜素 - 7 - O - 葡萄糖苷和绿原酸，表明它们有开发成新型口服抗癌和抗菌剂的潜力。