Aminzadeh Khosroshahi Mohaddeseh, Farrokhi Ali, Soltanzadeh Hossein
Department of Cellular and Molecular Biology, Bonab Branch, Islamic Azad University, Bonab, Iran.
Medicinal Plants Research Center, Maragheh University of Medical Sciences, Maragheh, Iran.
Addict Health. 2025 Jan;17:1484. doi: 10.34172/ahj.1484. Epub 2025 Jun 22.
Besides its physical and psychological effects on individuals, addiction is a major personal and societal issue that threatens cultural, political, and community well-being. Genetic factors play essential roles in susceptibility to methamphetamine dependence. The purpose of this study was to investigate the correlation between methamphetamine use disorder in Iranian males and the rs2652511 polymorphism in the promoter of SLC6A3.
We recruited 100 men with methamphetamine use disorder as cases and 100 age- and ethnically-matched normal men from East Azerbaijan, Tabriz, Iran as healthy controls. From peripheral blood leukocytes, genomic DNA was extracted. PCR-RFLP was utilized for genotyping.
The genotype distribution of rs2652511 polymorphism in the case group was 56% CC, 33% CT, and 11% 44, whereas in the control group it was 25% CC, 42% CT, and 33% TT. According to statistical analysis, there was a substantial variation in genotype and allele frequencies of the rs2652511 polymorphism between the case group and the healthy control group (>0.05).
Our research revealed that the rs2652511 polymorphism in the SLC6A3 gene was associated with methamphetamine misuse disorder in the Iranian population. To clarify the exact role of this polymorphism in the pathology of methamphetamine use disorder, further research is required across different racial and geographic groups.
成瘾除了对个人产生生理和心理影响外，还是一个重大的个人和社会问题，威胁着文化、政治和社区的福祉。遗传因素在甲基苯丙胺依赖易感性中起着重要作用。本研究的目的是调查伊朗男性甲基苯丙胺使用障碍与溶质载体家族6成员3（SLC6A3）启动子中rs2652511多态性之间的相关性。
我们招募了100名患有甲基苯丙胺使用障碍的男性作为病例，并从伊朗大不里士东阿塞拜疆省招募了100名年龄和种族匹配的正常男性作为健康对照。从外周血白细胞中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）进行基因分型。
病例组中rs2652511多态性的基因型分布为56%CC、33%CT和11%TT，而对照组中为25%CC、42%CT和33%TT。根据统计分析，病例组和健康对照组之间rs2652511多态性的基因型和等位基因频率存在显著差异（P>0.05）。
我们的研究表明，SLC6A3基因中的rs2652511多态性与伊朗人群中的甲基苯丙胺滥用障碍有关。为了阐明这种多态性在甲基苯丙胺使用障碍病理中的确切作用，需要在不同种族和地理群体中进行进一步研究。
