de Cezar Ana Luiza Molina, Freitas Rafaela Cristina, Neumann Amanda, Janegitz Bruno Campos
Laboratory of Sensors, Nanomedicine, and Nanostructured Materials, Universidade Federal de São Carlos, Araras, São Paulo 13604-900, Brasil.
ACS Meas Sci Au. 2025 Jul 18;5(4):572-580. doi: 10.1021/acsmeasuresciau.5c00056. eCollection 2025 Aug 20.
Screen-printed electrodes (SPEs) are an innovative technology in electrochemical sensors, offering advantages such as easy fabrication, large-scale production, low cost, and potential for miniaturization. These electrodes can be disposable and customized for various applications. Due to these advantages, SPEs are gaining attention in fields such as medicine and pharmacy. In this study, an electrochemical sensor was developed through screen-printing, using new conductive ink, compounded with carbon black, Chinese shellac, and acetone. The device was characterized by different approaches to analyze its characteristics, including scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetry, and contact angle. Also, the electrochemical characterizations were performed by using cyclic voltammetry and impedance spectroscopy. The sensor was employed to detect melatonin, a sleep-regulating hormone, and, under optimized parameters, the analytical curve by differential pulse voltammetry exhibited a linear range from 1.0 to 100 μmol L, with a limit of detection of 0.1 μmol L. The device was applied to synthetic urine samples using the addition and recovery method, yielding recovery values from 86.7 to 110%. The results indicate that the conductive ink is suitable for manufacturing printed electrodes, and the device proved promising for melatonin detection.
丝网印刷电极（SPEs）是电化学传感器领域的一项创新技术，具有易于制造、可大规模生产、成本低以及具备小型化潜力等优点。这些电极可以是一次性的，并且能够针对各种应用进行定制。由于这些优势，SPEs在医学和药学等领域正受到关注。在本研究中，通过丝网印刷，使用由炭黑、虫胶和丙酮混合而成的新型导电油墨，开发了一种电化学传感器。该装置通过不同方法进行表征以分析其特性，包括扫描电子显微镜、傅里叶变换红外光谱、X射线衍射、热重分析和接触角测量。此外，通过循环伏安法和阻抗谱进行电化学表征。该传感器用于检测褪黑素，一种调节睡眠的激素，在优化参数下，差分脉冲伏安法的分析曲线显示线性范围为1.0至100 μmol/L，检测限为0.1 μmol/L。使用加样回收法将该装置应用于合成尿液样本，回收率为86.7%至110%。结果表明，该导电油墨适用于制造印刷电极，并且该装置在褪黑素检测方面显示出前景。
