波兰西里西亚医院中产毒菌株的优势地位及新出现的PCR核糖体分型955的出现。
Dominance of toxigenic strains and the appearance of the emerging PCR ribotype 955 in hospitals in Silesia, Poland.
作者信息
Szarek Klaudia, Frankowska Natalia, Kabała Monika, Smits Wiep Klaas, Wultańska Dorota, Lalowski Piotr, Pituch Hanna, Iwanicki Adam, Hinc Krzysztof, Harmanus Céline, Sanders Ingrid M J G, Wojtyczka Robert, Wąsik Tomasz J
机构信息
Department of Medical Microbiology, Faculty of Medical Science in Katowice, Medical University of Silesia, Katowice, Poland.
Division of Molecular Bacteriology, Medical University of Gdańsk, Gdańsk, Poland.
出版信息
Front Microbiol. 2025 Aug 11;16:1644051. doi: 10.3389/fmicb.2025.1644051. eCollection 2025.
INTRODUCTION
Toxigenic strains of are the leading cause of healthcare-associated bacterial infections in Poland. In the Silesian Voivodeship, with 4.5 million inhabitants, the incidence of infection (CDI) in 2023 reached 65.1/100,000, slightly down from 70.0/100,000 in 2022. This study aimed to investigate the occurrence of toxigenic ribotypes in hospitalized patients in Silesia.
MATERIALS AND METHODS
A total of 130 stool samples from patients with confirmed antibiotic-associated diarrhea were tested. Multiplex PCR detected genes for GDH, toxins A/B, binary toxin CDT, and 16S rDNA. Ribotyping was performed by capillary PCR, and antibiotic susceptibility was tested with ETEST for 10 antibiotics.
RESULTS
Multiplex PCR confirmed the presence of all toxins (ABCDT) in 97 isolates. Six strains (6%) represented ribotype 955 (RT955), first reported in Poland in 2023. RT027 remained dominant (60%, = 78). All isolates were susceptible to vancomycin, while 4% (5/130) showed metronidazole resistance.
CONCLUSION
RT027 remains prevalent among CDI cases in Silesia. The emergence of RT955, closely related to a UK epidemic strain, suggests a possible shared origin and epidemiological link.
引言
在波兰，产毒艰难梭菌菌株是医疗保健相关细菌感染的主要原因。在拥有450万居民的西里西亚省，2023年艰难梭菌感染（CDI）的发病率达到65.1/10万，略低于2022年的70.0/10万。本研究旨在调查西里西亚住院患者中产毒艰难梭菌核糖型的发生情况。
材料与方法
对130例确诊为抗生素相关性腹泻患者的粪便样本进行检测。多重PCR检测了谷氨酸脱氢酶（GDH）、毒素A/B、二元毒素CDT和16S核糖体DNA（rDNA）的基因。通过毛细管PCR进行核糖分型，并用ETEST检测10种抗生素的药敏情况。
结果
多重PCR证实97株分离株中均存在所有毒素（ABCDT）。6株菌株（6%）代表核糖型955（RT955），于2023年在波兰首次报道。RT027仍然占主导地位（60%，n = 78）。所有分离株对万古霉素敏感，而4%（5/130）表现出甲硝唑耐药。
结论
RT027在西里西亚的CDI病例中仍然普遍存在。与英国流行菌株密切相关的RT955的出现表明可能存在共同起源和流行病学联系。
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