Yang Juan, Brilian Albertus Ivan, Shin Kwanwoo, Lee Yunmi, Jang Soojin, Jung Seong-Mook, Son Young Min, Park Kui Young, LeibundGut-Landmann Salomé, Jung Won Hee
Department of Systems Biotechnology, Chung-Ang University, Anseong, 17546, Korea.
Department of Chemistry and Institute of Biological Interfaces, Sogang University, Seoul, 04107 Korea.
Med Mycol. 2025 Sep 2;63(9). doi: 10.1093/mmy/myaf079.
The human skin is a crucial defense system, protecting against external stressors. However, the skin also hosts various microorganisms that impact skin health and disease. Therefore, the polymicrobial interaction in the skin is particularly interesting since it can significantly influence alterations in the virulence traits of microbes and the immune responses of the hosts. This study aimed to investigate the influence of Malassezia restricta, a predominant fungal species on human skin, on the virulence of Staphylococcus aureus, a prominent skin bacterium associated with atopic dermatitis. Our findings revealed that M. restricta effectively interferes with the invasion of S. aureus into human keratinocytes, suggesting a potential mechanism for influencing bacterial infection by the fungus. Additionally, we observed that M. restricta exhibits fibronectin binding capabilities, a key mediator in the S. aureus invasion of keratinocytes. Physicochemical analysis indicated the involvement of a heat-unstable component, likely a M. restricta cell surface protein, which necessitates physical contact between the fungus and keratinocytes for fibronectin binding. Collectively, our results suggest the influential role of M. restricta in the pathogenesis of S. aureus and reveal a novel aspect of this fungal species within the human skin microbial community.
人类皮肤是一个至关重要的防御系统，可抵御外部应激源。然而，皮肤中也存在各种微生物，它们会影响皮肤健康和疾病。因此，皮肤中的多微生物相互作用特别引人关注，因为它会显著影响微生物毒力特征的改变以及宿主的免疫反应。本研究旨在调查人类皮肤上主要的真菌物种——限制马拉色菌对金黄色葡萄球菌毒力的影响，金黄色葡萄球菌是一种与特应性皮炎相关的重要皮肤细菌。我们的研究结果表明，限制马拉色菌有效地干扰了金黄色葡萄球菌对人角质形成细胞的侵袭，这表明该真菌影响细菌感染的一种潜在机制。此外，我们观察到限制马拉色菌具有纤连蛋白结合能力，这是金黄色葡萄球菌侵袭角质形成细胞的关键介质。物理化学分析表明，一种热不稳定成分参与其中，可能是限制马拉色菌的细胞表面蛋白，它需要真菌与角质形成细胞之间的物理接触才能进行纤连蛋白结合。总体而言，我们的结果表明限制马拉色菌在金黄色葡萄球菌的发病机制中具有重要作用，并揭示了该真菌物种在人类皮肤微生物群落中的一个新方面。
