• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

新西兰白化兔视网膜玻璃体内联合及复合应用抗真菌药物的超微结构和免疫组织化学研究：一项实验方案

Ultrastructural and Immunohistochemical Study of Double and Combined Intravitreal Administration of Antifungal Agents in the Retina of New Zealand Albino Rabbits: An Experimental Protocol.

作者信息

Karachrysafi Sofia, Kourti Maria, Tsokkou Sophia, Ioannou Despoina, Kofidou Evangelia, Delis Georgios, Sotiriou Sotiris, Karamitsos Athanasios, Xioteli Maria, Dori Ioanna, Anastasiadou Penelope, Konstantinidis Ioannis, Kavvadas Dimitrios, Chatzinikolaou Fotios, Komnenou Anastasia, Karampatakis Vasileios, Sioga Antonia, Papamitsou Theodora

机构信息

Research Team "Histologistas", Interinstitutional Postgraduate Program "Health and Environmental Factors", Department of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Laboratory of Histology-Embryology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

出版信息

J Fungi (Basel). 2025 Jul 29;11(8):564. doi: 10.3390/jof11080564.

DOI:10.3390/jof11080564
PMID:
40863515
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12387355/
Abstract

INTRODUCTION

Fungal endophthalmitis (FE) is a rare but serious intraocular inflammatory disorder, resulting from an infection of the vitreous cavity from either endogenous or exogenous components that ultimately results in blindness. This current research study aims to elucidate the histological effects of the intravitreal injection of the maximum safe dosage of voriconazole and micafungin on the retina and investigate potential histological alterations after the double and combined administration of voriconazole and micafungin.

METHODOLOGY

Nine New Zealand Albino Rabbits were randomly assigned into three groups (V2, M2, and VM), and in each, voriconazole, micafungin, and a combination of the two medications were administered respectively. After the administration of the antifungal agents, the animals were sacrificed and their retinas were retrieved and studied under optical and electron microscopes. The immunohistochemical markers TNF-a and IL6 were also studied.

RESULTS

TNF-a was positive in the VM group, as it was found to be mildly positive in the presence of apoptotic cells in the ganglion cell layer.

CONCLUSIONS

This study revealed voriconazole has a greater toxicity in a multi-dosage administration. However, micafungin revealed a greater toxicity than voriconazole from the extent of the lesions observed.

摘要

引言

真菌性眼内炎（FE）是一种罕见但严重的眼内炎症性疾病，由内源性或外源性成分感染玻璃体腔引起，最终可导致失明。本研究旨在阐明玻璃体内注射伏立康唑和米卡芬净最大安全剂量对视网膜的组织学影响，并研究伏立康唑和米卡芬净联合及合并给药后的潜在组织学改变。

方法

将9只新西兰白化兔随机分为三组（V2、M2和VM），每组分别给予伏立康唑、米卡芬净以及两种药物的组合。给予抗真菌药物后，处死动物，取出视网膜，在光学显微镜和电子显微镜下进行研究。还研究了免疫组化标志物肿瘤坏死因子-α（TNF-α）和白细胞介素6（IL6）。

结果

VM组中TNF-α呈阳性，因为在神经节细胞层存在凋亡细胞时发现其呈轻度阳性。

结论

本研究表明，多剂量给药时伏立康唑具有更大的毒性。然而，从观察到的病变程度来看，米卡芬净显示出比伏立康唑更大的毒性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/1376c127384d/jof-11-00564-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/d460ff899f7a/jof-11-00564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/9a3ee5877cfa/jof-11-00564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/68b653f91edc/jof-11-00564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/81a3c0ed7925/jof-11-00564-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/a388c3d2ceb4/jof-11-00564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/0ecb13972e23/jof-11-00564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/887b4f355249/jof-11-00564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/0e86aa1fb98c/jof-11-00564-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/efbfa6906759/jof-11-00564-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/cbd6abf75cfe/jof-11-00564-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/1376c127384d/jof-11-00564-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/d460ff899f7a/jof-11-00564-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/9a3ee5877cfa/jof-11-00564-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/68b653f91edc/jof-11-00564-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/81a3c0ed7925/jof-11-00564-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/a388c3d2ceb4/jof-11-00564-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/0ecb13972e23/jof-11-00564-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/887b4f355249/jof-11-00564-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/0e86aa1fb98c/jof-11-00564-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/efbfa6906759/jof-11-00564-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/cbd6abf75cfe/jof-11-00564-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/221f/12387355/1376c127384d/jof-11-00564-g011.jpg

相似文献

1
Ultrastructural and Immunohistochemical Study of Double and Combined Intravitreal Administration of Antifungal Agents in the Retina of New Zealand Albino Rabbits: An Experimental Protocol.新西兰白化兔视网膜玻璃体内联合及复合应用抗真菌药物的超微结构和免疫组织化学研究：一项实验方案
J Fungi (Basel). 2025 Jul 29;11(8):564. doi: 10.3390/jof11080564.
2
Prescription of Controlled Substances: Benefits and Risks管制药品的处方：益处与风险
3
Home treatment for mental health problems: a systematic review.心理健康问题的居家治疗：一项系统综述
Health Technol Assess. 2001;5(15):1-139. doi: 10.3310/hta5150.
4
Adjunctive steroid therapy versus antibiotics alone for acute endophthalmitis after intraocular procedure.辅助性类固醇疗法与单纯抗生素疗法治疗眼内手术后急性眼内炎的对比
Cochrane Database Syst Rev. 2017 Feb 22;2(2):CD012131. doi: 10.1002/14651858.CD012131.pub2.
5
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.慢性斑块状银屑病的全身药理学治疗：一项网状Meta分析。
Cochrane Database Syst Rev. 2020 Jan 9;1(1):CD011535. doi: 10.1002/14651858.CD011535.pub3.
6
The Black Book of Psychotropic Dosing and Monitoring.《精神药物剂量与监测黑皮书》
Psychopharmacol Bull. 2024 Jul 8;54(3):8-59.
7
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.系统性药理学治疗慢性斑块状银屑病：网络荟萃分析。
Cochrane Database Syst Rev. 2021 Apr 19;4(4):CD011535. doi: 10.1002/14651858.CD011535.pub4.
8
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.慢性斑块状银屑病的全身药理学治疗：一项网状荟萃分析。
Cochrane Database Syst Rev. 2017 Dec 22;12(12):CD011535. doi: 10.1002/14651858.CD011535.pub2.
9
A rapid and systematic review of the clinical effectiveness and cost-effectiveness of topotecan for ovarian cancer.拓扑替康治疗卵巢癌的临床有效性和成本效益的快速系统评价。
Health Technol Assess. 2001;5(28):1-110. doi: 10.3310/hta5280.
10

本文引用的文献

1
The Diagnosis and Treatment of Fungal Endophthalmitis: An Update.真菌性眼内炎的诊断与治疗：最新进展
Diagnostics (Basel). 2022 Mar 10;12(3):679. doi: 10.3390/diagnostics12030679.
2
Fungal Endophthalmitis: Analysis of 730 Consecutive Eyes from 7 Tertiary Eye Care Centers in India.真菌性眼内炎：来自印度7家三级眼科护理中心的730例连续病例分析。
Ophthalmol Retina. 2022 Mar;6(3):243-251. doi: 10.1016/j.oret.2021.09.006. Epub 2021 Sep 20.
3
Endogenous Endophthalmitis: A Review of Case Series Published between 2011 and 2020.内源性眼内炎：2011年至2020年间发表的病例系列综述
J Ophthalmol. 2020 Oct 23;2020:8869590. doi: 10.1155/2020/8869590. eCollection 2020.
4
Histological Effects of Intravitreal Injection of Antifungal Agents in New Zealand White Rabbits: An Electron Microscopic and Immunohistochemical Study.玻璃体内注射抗真菌药物对新西兰白兔的组织学影响：一项电子显微镜和免疫组织化学研究。
Pharmaceuticals (Basel). 2020 Sep 23;13(10):267. doi: 10.3390/ph13100267.
5
The Role of Systemic Antimicrobials in the Treatment of Endophthalmitis: A Review and an International Perspective.全身用抗菌药物在眼内炎治疗中的作用：综述与国际视角
Ophthalmol Ther. 2020 Sep;9(3):485-498. doi: 10.1007/s40123-020-00270-w. Epub 2020 Jul 1.
6
RISK FACTORS, TREATMENT STRATEGIES, AND OUTCOMES OF ENDOPHTHALMITIS ASSOCIATED WITH SEVERE FUNGAL KERATITIS.严重真菌性角膜炎相关眼内炎的危险因素、治疗策略和转归。
Retina. 2019 Jun;39(6):1076-1082. doi: 10.1097/IAE.0000000000002112.
7
Endophthalmitis.眼内炎
Korean J Ophthalmol. 2017 Aug;31(4):283-289. doi: 10.3341/kjo.2017.0036. Epub 2017 Jun 28.
8
Echinocandins in Ocular Therapeutics.棘白菌素类药物在眼科治疗中的应用
J Ocul Pharmacol Ther. 2017 Jun;33(5):340-352. doi: 10.1089/jop.2016.0186. Epub 2017 Feb 24.
9
The Role of Vitrectomy in the Management of Fungal Endophthalmitis.玻璃体切除术在真菌性眼内炎治疗中的作用
Semin Ophthalmol. 2017;32(1):29-35. doi: 10.1080/08820538.2016.1228396. Epub 2016 Oct 28.
10
Cytokine Regulation of Metastasis and Tumorigenicity.细胞因子对转移和肿瘤发生的调控。
Adv Cancer Res. 2016;132:265-367. doi: 10.1016/bs.acr.2016.05.005. Epub 2016 Aug 5.