Vidal-Montiel Alvaro, Clark-Flores Daniel, Valentín-Gómez Eulogio, Luna-Arias Juan Pedro, Rosales-Cruz Erika, Hernández-Rodríguez César, Villa-Tanaca Lourdes, Juárez-Montiel Margarita
Laboratorio de Biología Molecular de Bacterias y Levaduras, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tomás, Mexico City 11340, Mexico.
Departamento de Microbiología y Ecología, Universidad de Valencia, 46100 Burjassot, Valencia, Spain.
J Fungi (Basel). 2025 Aug 1;11(8):573. doi: 10.3390/jof11080573.
is a multidrug-resistant, thermo- and osmotolerant yeast capable of persisting on biotic and abiotic surfaces, attributes likely linked to its cell wall composition. Here, seven putative genes encoding yapsins, aspartyl proteases GPI-anchored to the membrane or cell wall, were identified in the genomes of CJ97 and 20-1498, from clades III and IV, respectively. The gene is orthologous to the of . The gene is orthologous to in and , so that they may share similar roles. An in silico analysis suggested an interaction between pepstatin and the catalytic domain of Yps1 and Yps7. Although this inhibitor, when combined with caffeine, had a subtle effect on the growth of , it induced alterations in the cell wall. and expression increased under nutrient starvation and NaCl, and at 42 °C. The transcriptome of the 20-1498 strain suggests that autophagy may play a role in thermal stress, probably degrading deleterious proteins or maintaining cell wall and vacuolar homeostasis. Therefore, CauYps1 and CauYps7 may play a role in the cell wall integrity of in stress conditions, and they could be a target of new antifungal or antivirulence agents.
是一种耐多药、耐热和耐渗透压的酵母，能够在生物和非生物表面存活，这些特性可能与其细胞壁组成有关。在这里，在分别来自进化枝III和IV的CJ97和20 - 1498的基因组中鉴定出七个推定的编码yapsins的基因，yapsins是锚定在膜或细胞壁上的天冬氨酸蛋白酶。该基因与的是直系同源基因。该基因与和中的是直系同源基因，因此它们可能具有相似的作用。计算机模拟分析表明胃蛋白酶抑制剂与Yps1和Yps7的催化结构域之间存在相互作用。尽管这种抑制剂与咖啡因联合使用时对的生长有微妙的影响，但它会引起细胞壁的改变。在营养饥饿、NaCl处理以及42℃条件下，和的表达增加。20 - 1498菌株的转录组表明自噬可能在热应激中起作用，可能是降解有害蛋白质或维持细胞壁和液泡的稳态。因此，CauYps1和CauYps7可能在应激条件下的细胞壁完整性中起作用，并且它们可能成为新型抗真菌或抗毒力药物的靶点。
