Dupraz Marlène, Lancelot Renaud, Diouf Gorgui, Malfacini Marco, Marquereau Lucie, Gouagna Louis-Clément, Rossignol Marie, Chandre Fabrice, Baldet Thierry, Bouyer Jérémy
UMR ASTRE, CIRAD-INRAE-Univ. Montpellier, Montpellier 34398, France - UMR ASTRE, CIRAD-INRAE-Univ. Montpellier, Plateforme CYROI, Sainte Clotilde 97491, La Réunion, France.
Laboratoire National d'Elevage et de Recherches Vétérinaires, ISRA, BP 2057, Dakar, Sénégal.
Parasite. 2025;32:55. doi: 10.1051/parasite/2025047. Epub 2025 Aug 27.
Innovative control tools are needed against Aedes mosquitoes. The boosted sterile insect technique (bSIT) consists of treating sterile males with a biocide prior to their release to contaminate larval habitats. We compared the efficacy of SIT and boosted SIT to prevent the emergence of adult Aedes albopictus in large cages. We tested two sterile-to-fertile male ratios: 5:1 (SIT5) and 1:1 (SIT1), with and without pyriproxyfen enhancement (bSIT or SIT). The eggs were collected in ovitraps and the immature stages were monitored until adult emergence or up to 15 days after hatching to estimate the relative risk (RR) of adult emergence compared to the control category. The concentration of pyriproxyfen in the ovitrap water did not change when sterile males were released with females or alone (χ = 0.99, df = 1, p = 0.547). This concentration was higher when the sterile-to-fertile male ratio was increased from 1:1 to 5:1: χ = 18.8, df = 1, p = 0.006. All four treatment categories were effective in suppressing mosquito populations. With a relative risk RR = 0.194 95% CI [0.128; 0.275], SIT5 was the most effective. Boosted SIT was not as effective as SIT. However, bSIT1 (RR = 0.418 [0.351; 0.492]) and bSIT5 (RR = 0.512 [0.431; 0.596]) were equally effective. Boosted males directly vectored pyriproxyfen to breeding sites. Boosted SIT was more effective than SIT alone with a low sterile-to-fertile male ratio. Under operational conditions, it could be initially deployed to suppress the target population and then switched to standard SIT.
需要创新的控制工具来对付伊蚊。增强型不育昆虫技术（bSIT）包括在释放不育雄蚊之前用杀生物剂对其进行处理，以污染幼虫栖息地。我们比较了昆虫不育技术（SIT）和增强型昆虫不育技术在大型笼子中防止白纹伊蚊成虫羽化的效果。我们测试了两种不育雄蚊与可育雄蚊的比例：5:1（SIT5）和1:1（SIT1），有和没有吡丙醚增强处理（bSIT或SIT）。在诱蚊产卵器中收集卵，并监测未成熟阶段，直到成虫羽化或孵化后15天，以估计与对照类别相比成虫羽化的相对风险（RR）。当不育雄蚊与雌蚊一起或单独释放时，诱蚊产卵器水中吡丙醚的浓度没有变化（χ = 0.99，自由度 = 1，p = 0.547）。当不育雄蚊与可育雄蚊的比例从1:1增加到5:1时，该浓度更高：χ = 18.8，自由度 = 1，p = 0.006。所有四个处理类别在抑制蚊子种群方面均有效。相对风险RR = 0.194，95%置信区间[0.128；0.275]，SIT5最有效。增强型SIT不如SIT有效。然而，bSIT1（RR = 0.418 [0.351；0.492]）和bSIT5（RR = 0.512 [0.431；0.596]）同样有效。增强型雄蚊将吡丙醚直接传播到繁殖地点。在不育雄蚊与可育雄蚊比例较低时，增强型SIT比单独的SIT更有效。在实际操作条件下，它可以首先用于抑制目标种群，然后切换到标准的昆虫不育技术。
