Danielisová V, Chavko M, Kehr J
Neurochem Res. 1987 Mar;12(3):241-5. doi: 10.1007/BF00972133.
Rabbit spinal cords were subjected to 10 to 40 minutes of ischemia with and without 4 days of recirculation and L-4 segment was analyzed for adenylates and ATP-induced bioluminiscence. ATP level and energy charge was progressively reduced by increasing durations of ischemia. Regional evaluation of ATP-induced bioluminiscence after 10 and 20 minutes of ischemia revealed ATP depletion mainly in the gray matter of spinal cord. Forty minutes of ischemia resulted in complete reduction of ATP bioluminiscence in both gray and white matter. Within 4 days of recirculation following all periods of ischemia studied, only partial metabolic recovery occurred. Restitution of ATP-induced bioluminiscence was regionally heterogeneous, reduced predominantly in the anterior horns of gray matter.
对兔脊髓进行10至40分钟的缺血处理，部分伴有4天的再灌注，然后分析L-4节段的腺苷酸和ATP诱导的生物发光。随着缺血时间的延长，ATP水平和能荷逐渐降低。缺血10分钟和20分钟后对ATP诱导的生物发光进行区域评估，结果显示ATP耗竭主要发生在脊髓灰质。40分钟的缺血导致灰质和白质中ATP生物发光完全消失。在所有研究的缺血期后的4天再灌注内，仅发生了部分代谢恢复。ATP诱导的生物发光恢复在区域上是不均匀的，主要在灰质前角降低。
