Ozdemir Ozgur, Duygulu Mustafa Emre, Tekelioglu Yavuz, Ersoz Safak, Guven Suleyman
Independent Researcher, Trabzon 61050, Turkey.
Department of Medical Oncology, Faculty of Medicine, Karadeniz Technical University, Trabzon 61080, Turkey.
Cancers (Basel). 2025 Aug 19;17(16):2691. doi: 10.3390/cancers17162691.
: Flow cytometric analysis of cellular DNA content has prognostic importance in ovarian cancer and its measurement could contribute to clinical practice. The aim of this study was to compare serous cystadenoma and serous cystadenocarcinoma cases in terms of their flow cytometric analysis results. : In total, 60 serous ovarian tumor cases (30 cases of ovarian serous cyst adenoma and 30 cases of ovarian serous cystadenocarcinoma) in paraffin blocks were extracted from hospital pathology archives for flow cytometric analysis. Cell cycle changes, aneuploidy ratio, and annexin V levels were compared. : The S phase cell ratio, proliferative index, aneuploidy cell ratio, and annexin V apoptotic index were significantly higher in the cancer group compared to the cystadenoma group. However, the G2/M phase cell ratio was found to be similar in both groups. When the annexin V apoptotic index cutoff value was selected as 27.65%, the sensitivity was calculated as 90.0% and the specificity as 93.3% for predicting serous ovarian carcinoma (AUC, 0.872; < 0.001; 95% CI, 0.761-0.984). : In serous cystadenocarcinoma cases, cell cycle activity was increased compared to serous cyst adenoma, but the similarity of G2/M activities suggested that serous cystadenocarcinoma may be a member of the "metaneoplasia" process. The detection of annexin V expression in ovarian tumors by flow cytometric analysis is a cheap, fast, and easy diagnostic method that can be used in the diagnosis of ovarian cancer. Annexin V expression is an effective biomarker with high sensitivity and specificity that could be used in the diagnosis of serous ovarian cancer.
细胞DNA含量的流式细胞术分析在卵巢癌中具有预后意义，其检测有助于临床实践。本研究的目的是比较浆液性囊腺瘤和浆液性囊腺癌病例的流式细胞术分析结果。
共从医院病理档案中提取60例石蜡包埋的卵巢浆液性肿瘤病例（30例卵巢浆液性囊腺瘤和30例卵巢浆液性囊腺癌）进行流式细胞术分析。比较细胞周期变化、非整倍体率和膜联蛋白V水平。
与囊腺瘤组相比，癌症组的S期细胞比例、增殖指数、非整倍体细胞比例和膜联蛋白V凋亡指数显著更高。然而，发现两组的G2/M期细胞比例相似。当将膜联蛋白V凋亡指数临界值设定为27.65%时，预测浆液性卵巢癌的敏感性计算为90.0%，特异性为93.3%（AUC，0.872；P<0.001；95%CI，0.761 - 0.984）。
在浆液性囊腺癌病例中，与浆液性囊腺瘤相比细胞周期活性增加，但G2/M活性的相似性表明浆液性囊腺癌可能是“化生瘤变”过程的一员。通过流式细胞术分析检测卵巢肿瘤中膜联蛋白V的表达是一种廉价、快速且简便的诊断方法，可用于卵巢癌的诊断。膜联蛋白V表达是一种具有高敏感性和特异性的有效生物标志物，可用于浆液性卵巢癌的诊断。
