Filip Mara, Clichici Simona Valeria, Muntean Mara, David Luminița, Moldovan Bianca, Toma Vlad Alexandru, Login Cezar, Suciu Şoimița Mihaela
Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 1-3 Clinicilor Street, 400006 Cluj-Napoca, Romania.
Department of Cell and Molecular Biology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur Street, 400349 Cluj-Napoca, Romania.
Biomolecules. 2025 Jul 24;15(8):1068. doi: 10.3390/biom15081068.
The present study aimed to evaluate the therapeutic benefits of a hybrid material based on gold nanoparticles and natural extracts on an experimental model of thioacetamide-induced (TAA) liver injury in rats. The nanomaterials were synthesized using a green method, with L. extract as a reducing and capping agent (NPCS), and were then mixed with L. (VL) extract in order to achieve a final mixture with enhanced properties (NPCS-VL). NPCSs were characterized using UV-vis spectrophotometry and transmission electron microscopy (TEM), which demonstrated the formation of spherical, stable gold nanoparticles with an average diameter of 20 nm. NPCS-VL's hepatoprotective effects were evaluated through an analysis of oxidative stress, inflammation, hepatic cytolysis, histology assays, and TEM in comparison to silymarin on an animal model of thioacetamide (TAA)-induced toxic hepatitis. TAA administration determined hepatotoxicity, as it triggered redox imbalance, increased proinflammatory cytokine levels and alanine aminotransferase (ALAT) activity, and induced morphological and ultrastructural changes characteristic of liver fibrosis. In rats treated with NPCS-VL, all these pathological processes were attenuated, suggesting a potential antifibrotic effect of this hybrid bionanomaterial.
本研究旨在评估一种基于金纳米颗粒和天然提取物的混合材料对硫代乙酰胺（TAA）诱导的大鼠肝损伤实验模型的治疗效果。采用绿色方法合成纳米材料，以L.提取物作为还原剂和封端剂（NPCS），然后将其与L.（VL）提取物混合，以获得具有增强性能的最终混合物（NPCS-VL）。使用紫外可见分光光度法和透射电子显微镜（TEM）对NPCS进行表征，结果表明形成了平均直径为20nm的球形稳定金纳米颗粒。与水飞蓟宾相比，通过对硫代乙酰胺（TAA）诱导的中毒性肝炎动物模型进行氧化应激、炎症、肝细胞溶解、组织学分析和TEM分析，评估了NPCS-VL的肝保护作用。给予TAA会导致肝毒性，因为它引发了氧化还原失衡，增加了促炎细胞因子水平和丙氨酸转氨酶（ALAT）活性，并诱导了肝纤维化的形态学和超微结构变化。在用NPCS-VL治疗的大鼠中，所有这些病理过程均得到缓解，表明这种混合生物纳米材料具有潜在的抗纤维化作用。
