Gutierrez-Albanchez Enrique, Fuente-González Elena, Plokhovska Svitlana, Gutierrez-Mañero Francisco Javier, Ramos-Solano Beatriz
Faculty of Pharmacy, Universidad San Pablo-CEU Universities, 28040 Madrid, Spain.
Institute of Food Biotechnology and Genomics, NAS of Ukraine, 01112 Kyiv, Ukraine.
Antioxidants (Basel). 2025 Jul 29;14(8):931. doi: 10.3390/antiox14080931.
Rosemary () is renowned for its antioxidant, anti-inflammatory, and antihyperglycemic properties, largely attributed to its rich phytochemical profile. This study evaluates the potential of metabolites from NFV3, formulated in silver nanoparticles (AgNPs), to enhance the bioactivity of rosemary extracts in postharvest applications. Rosemary stems were treated with AgNPs coated with bacterial metabolites (NP), bacterial cells, or metabolites (LM), and the extracts' phytochemical composition and bioactivities were assessed. HPLC and HPLC-MS analyses revealed that the NP treatment induced significant metabolic remodeling, particularly upregulating rosmarinic acid and selected triterpenes (ursolic and betulinic acids), while reducing carnosic acid levels. NP-treated extracts exhibited significantly enhanced inhibition of cyclooxygenase (COX-1 and COX-2), indicating improved anti-inflammatory potential. The α-glucosidase inhibition and antioxidant activity (DPPH assay) of the extracts were not substantially altered, suggesting the selective enhancement of pharmacological functions. These findings demonstrate that nanoparticle-based elicitation selectively remodels secondary metabolism in rosemary, improving extract quality and bioactivity. This strategy offers a novel, sustainable tool for optimizing plant-based therapeutics in the phytopharmaceutical industry.
迷迭香（Rosemary）以其抗氧化、抗炎和降血糖特性而闻名，这在很大程度上归因于其丰富的植物化学成分。本研究评估了由银纳米颗粒（AgNPs）配制的NFV3的代谢产物在采后应用中增强迷迭香提取物生物活性的潜力。用涂有细菌代谢产物的AgNPs（NP）、细菌细胞或代谢产物（LM）处理迷迭香茎，并评估提取物的植物化学成分和生物活性。高效液相色谱（HPLC）和高效液相色谱-质谱（HPLC-MS）分析表明，NP处理引起了显著的代谢重塑，特别是上调了迷迭香酸和选定的三萜类化合物（熊果酸和桦木酸），同时降低了肌醇六磷酸水平。经NP处理的提取物对环氧化酶（COX-1和COX-2）的抑制作用显著增强，表明其抗炎潜力有所提高。提取物的α-葡萄糖苷酶抑制作用和抗氧化活性（DPPH测定）没有实质性改变，表明其药理功能有选择性增强。这些发现表明，基于纳米颗粒的诱导作用选择性地重塑了迷迭香中的次生代谢，提高了提取物的质量和生物活性。该策略为优化植物制药行业中基于植物的治疗方法提供了一种新颖、可持续的工具。
