Montalvo Grecia, Caballeros Sarabí, Escalante Karla, Barreto Alvaro, Arenas Martín, Gaxiola Gabriela
Unidad Multidisciplinaria de Docencia e Investigación (UMDI) Sisal, Facultad de Ciencias, Universidad Nacional Autónoma de México (UNAM), Yucatán, Hunucmá 97351, Mexico.
Instituto Tecnológico de Conkal, Yucatán, Mérida 97345, Mexico.
Antioxidants (Basel). 2025 Aug 12;14(8):988. doi: 10.3390/antiox14080988.
This study evaluated the effect of vitamin C (L-ascorbyl-2-polyphosphate) on the physiological condition, biochemical antioxidant activity, immune responses, and gene expression in the reproductive tract, as well as on sperm quantity and quality in male white shrimp broodstock. Four diets containing 42.5% protein, 11.5% lipids, and 23.5% carbohydrates were formulated with L-ascorbyl-2-polyphosphate as a source of vitamin C at the following concentrations: 0.016 g/kg (Basal), 0.322 g/kg (A), 0.628 g/kg (B), and 0.934 g/kg (). Shrimp fed diet exhibited the highest SOD and CAT activity and serum cholesterol levels, but the lowest expression of hemocyanin (Hemo) mRNA transcripts ( ˂ 0.05). Shrimp fed diet A showed the highest Hemo mRNA expression and phenoloxidase (PO) activity, while those fed diet B had the highest serum triglyceride levels ( ˂ 0.05). In contrast, shrimp fed diets A and B exhibited the lowest serum cholesterol levels ( ˂ 0.05). There were no differences in sperm quality between the diets. In relation to sperm quantity, the shrimp fed diet B had the highest sperm cell count (2,750,000 cel/mL), and those fed diet A had the lowest (585,000 cel/mL) ( ˂ 0.05). These results indicate that vitamin C influences the reproductive aspects of male broodstock. A dietary inclusion level of 0.628 g/kg promotes optimal physiological, oxidative stress, and immunological conditions for increased sperm cell production, whereas an excessive level may promote oxidative stress.
本研究评估了维生素C（L-抗坏血酸-2-多聚磷酸酯）对雄性凡纳滨对虾亲虾生理状况、生化抗氧化活性、免疫反应、生殖道基因表达以及精子数量和质量的影响。以L-抗坏血酸-2-多聚磷酸酯作为维生素C来源，配制了四种蛋白质含量为42.5%、脂质含量为11.5%、碳水化合物含量为23.5%的饲料，其维生素C浓度分别为：0.016 g/kg（基础组）、0.322 g/kg（A组）、0.628 g/kg（B组）和0.934 g/kg（组）。投喂组饲料的凡纳滨对虾表现出最高的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及血清胆固醇水平，但血蓝蛋白（Hemo）mRNA转录本的表达最低（P˂0.05）。投喂A组饲料的凡纳滨对虾Hemo mRNA表达和酚氧化酶（PO）活性最高，而投喂B组饲料的凡纳滨对虾血清甘油三酯水平最高（P˂0.05）。相比之下，投喂A组和B组饲料的凡纳滨对虾血清胆固醇水平最低（P˂0.05）。不同饲料组间精子质量无差异。在精子数量方面，投喂B组饲料的凡纳滨对虾精子细胞计数最高（2750000个细胞/毫升），而投喂A组饲料的凡纳滨对虾精子细胞计数最低（585000个细胞/毫升）（P˂0.05）。这些结果表明，维生素C会影响雄性凡纳滨对虾亲虾的生殖性能。饲料中维生素C添加水平为0.628 g/kg时，可促进精子细胞生成的最佳生理、氧化应激和免疫状况，而过量添加可能会促进氧化应激。
