Aobchey Paitoon, Chaiyawat Parunya, Seesen Mathuramat, Ruangsuriya Jetsada
Functional Food Research Center for Well-Being, The Multidisciplinary Research Institute, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Multidisciplinary Technology for Advanced Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Life (Basel). 2025 Aug 21;15(8):1330. doi: 10.3390/life15081330.
Cognitive frailty in elders has become very common in Thailand society and is extremely difficult to manage in clinical practice due to poor detection and diagnosis. Plasma proteomic studies may be able to provide promising protein markers specific to this condition in order to improve the quality of life in the elderly. The purpose of this study was to differentiate and identify a potential protein marker of cognitive frailty in plasma samples by proteomic approaches. Nine plasma samples from elders with cognitive frailty or non-frailty were pooled and subjected to 2D gel separation. Conventional analysis was performed with the stained gel, and the selected protein spot was identified by liquid chromatography-tandem mass spectrometry coupled to a SCIEX TripleTOF 6600+. It was found that fibrinogen gamma (FGG) chain protein was suggested. FGG was further validated with a commercial ELISA kit using both pooled and individual plasma samples. It was found that both pooled and individual samples showed a significant reduction in FGG levels in elders with cognitive frailty. The results from this study suggest that FGG chain may be a potentially useful plasma biomarker for early detection, diagnosis, and therapeutic applications of cognitive frailty in elders in order to achieve sustainable health in an aging society.
老年人的认知衰弱在泰国社会已变得非常普遍，并且由于检测和诊断不佳，在临床实践中极难管理。血浆蛋白质组学研究或许能够提供针对这种情况的有前景的蛋白质标志物，以改善老年人的生活质量。本研究的目的是通过蛋白质组学方法区分和鉴定血浆样本中认知衰弱的潜在蛋白质标志物。将来自认知衰弱或非衰弱老年人的9份血浆样本混合，并进行二维凝胶分离。对染色后的凝胶进行常规分析，通过与SCIEX TripleTOF 6600+联用的液相色谱-串联质谱法鉴定所选的蛋白质斑点。结果发现了纤维蛋白原γ（FGG）链蛋白。使用商业ELISA试剂盒，通过混合血浆样本和个体血浆样本对FGG进行了进一步验证。结果发现，无论是混合样本还是个体样本，认知衰弱老年人的FGG水平均显著降低。本研究结果表明，FGG链可能是一种潜在有用的血浆生物标志物，可用于老年人认知衰弱的早期检测、诊断和治疗应用，以在老龄化社会中实现可持续健康。
